Use of an Automatic DNA Sequencer for S1 Mapping: Transcriptional Analysis of the Streptomyces Coelicolor A3(2) Dnak Operon

Brans, Alain; Loriaux, Axelle; Thamm, Iris; Joris, Bernard; Dusart, Jean

doi:10.1016/S0378-1097(97)00074-8

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Use of an Automatic DNA Sequencer for S1 Mapping: Transcriptional Analysis of the Streptomyces Coelicolor A3(2) Dnak Operon

Brans, Alain; Loriaux, Axelle; Thamm, Iris et al.

1997 • In FEMS Microbiology Letters, 149 (2), p. 189-94

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https://hdl.handle.net/2268/1780

DOI
10.1016/S0378-1097(97)00074-8

PubMed
9141660

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Abstract :

[en] The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Brans, Alain ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Loriaux, Axelle; Université de Liège - ULiège > Centre d'ingénierie des protéines

Thamm, Iris ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Joris, Bernard ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Dusart, Jean; Université de Liège - ULiège > Centre d'Ingénierie des Protéines

Language :

English

Title :

Use of an Automatic DNA Sequencer for S1 Mapping: Transcriptional Analysis of the Streptomyces Coelicolor A3(2) Dnak Operon

Publication date :

15 April 1997

Journal title :

FEMS Microbiology Letters

ISSN :

0378-1097

eISSN :

1574-6968

Publisher :

Oxford University Press, United Kingdom

Volume :

149

Issue :

Pages :

189-94

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 02 December 2008

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Bibliography

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