Article (Scientific journals)
Effects of prolactin deficiency on myelopoiesis and splenic T lymphocyte proliferation in thermally injured mice.
Dugan, Amy L.; Thellin, Olivier; Buckley, Donna J. et al.
2002In Endocrinology, 143 (10), p. 4147-4151
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Abstract :
[en] The importance of prolactin (PRL) in mammopoiesis and milk production is undisputed. However, previous studies investigating the role of PRL in immune function have yielded inconsistencies. These inconsistencies have led to our hypothesis that the immunomodulatory effects of PRL are only manifest under conditions in which the organism is subjected to stress. Thermal injury is a well-known stressor. The goal of this study was to determine whether the lack of PRL enhanced the negative effects of thermal injury-induced immune alterations utilizing a mouse model in which the PRL gene had been disrupted. Mice received either sham or burn treatment, and were sacrificed 4 days later. The immune parameters studied were the capacity of bone marrow cells to form granulocyte-macrophage colony forming units (GM-CFU) in the presence of granulocyte-macrophage colony stimulating factor, and the ability of the splenic T lymphocytes to proliferate in response to phytohemagglutin (PHA). As shown by others, our results reveal that burn increased the number of GM-CFU compared to sham controls; however, this elevation was only significant in the PRL-/- mice. Thermal injury increased PHA-stimulated proliferation of splenic T lymphocytes, however this increase was only significant in the PRL+/- group. We conclude that under conditions of a controlled stress event (thermal injury) [a] the increase in the GM-CFU is exaggerated in the absence of PRL, and [b] the enhancement of PHA-induced proliferation of splenic lymphocytes required PRL. This study supports the hypothesis that the immunomodulatory effects of PRL are manifest when the organism is subjected to stress.
Research center :
University of Cincinnati (OH, USA), Department of Molecular and Cellular Physiology
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Dugan, Amy L.;  University of Cincinnati (OH, USA) > Department of Molecular and Cellular Physiology
Thellin, Olivier ;  University of Cincinnati (OH, USA) > Department of Molecular and Cellular Physiology
Buckley, Donna J.;  University of Cincinnati (OH, USA) > College of Pharmacy
Buckley, Arthur R.;  University of Cincinnati (OH, USA) > College of Pharmacy
Ogle, Cora K.;  Shriners Hospital for Children, Cincinnati (OH, USA)
Horseman, Nelson D.;  University of Cincinnati (OH, USA) > Department of Molecular and Cellular Physiology
Language :
English
Title :
Effects of prolactin deficiency on myelopoiesis and splenic T lymphocyte proliferation in thermally injured mice.
Publication date :
2002
Journal title :
Endocrinology
ISSN :
0013-7227
eISSN :
1945-7170
Publisher :
Endocrine Society, Chevy Chase, United States - Maryland
Volume :
143
Issue :
10
Pages :
4147-4151
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 January 2015

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