Reference : Morphological and molecular characterization of planktonic cyanobacteria from Belgium...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Life sciences : Aquatic sciences & oceanology
Morphological and molecular characterization of planktonic cyanobacteria from Belgium and Luxembourg
Willame, R. [> > > >]
Boutte, C. [> > > >]
Grubisic, Stana mailto [Université de Liège - ULiège > Services administratifs généraux > Protection et hygiène du travail (SUPHT) >]
Wilmotte, Annick mailto [Université de Liège - ULiège > Département des sciences de la vie > Enzymologie >]
Komarek, J. [> > > >]
Hoffmann, L. [> > > >]
Journal of Phycology
Blackwell Publishing
Yes (verified by ORBi)
[en] 16S rRNA gene ; bloom ; freshwater cyanobacteria ; nanoplankton ; periphyton ; phytobenthos ; picoplankton ; polyphasic taxonomy
[en] For the first time in Belgium and Luxembourg, the diversity and taxonomy of 95 cyanobacterial strains isolated from freshwater blooms were assessed by the comparison of phenotypes and partial 16S rRNA gene sequences. The results showed the high diversity of nanoplanktonic, picoplanktonic, and benthic-periphytic cyanobacteria accompanying the main bloom-forming taxa. Indeed, besides 15 morphotypes of bloom-forming taxa, seven non-bloom-forming planktonic morphotypes and 11 morphotypes from benthic-periphytic taxa were isolated in culture from the plankton samples of 35 water bodies. The bloom-forming strains belonged to the genera Microcystis, Woronichinia, Planktothrix, Anabaena, and Aphanizomenon, whereas the other strains isolated from the same samples were assigned to the nanoplanktonic Aphanocapsa, Aphanothece, Snowella, and Pseudanabaena; to the picoplanktonic Cyanobium; and to the benthic periphytic Geitlerinema, Komvophoron, Leptolyngbya, Lyngbya, Phormidium, Calothrix, Nostoc, and Trichormus. The results supported both the polyphyletism of genera such as Aphanocapsa, Aphanothece, Leptolyngbya, Geitlerinema, Anabaena, and Aphanizomenon as well as the validity of genera such as Microcystis, Planktothrix, and Pseudanabaena with gas vesicles and cells constricted at the cross wall. The results obtained showed the close relationship between Snowella and Woronichinia for which very few sequences exist. The first sequence of Komvophoron appeared poorly related to other available cyanobacterial sequences. Although in a few cases a good agreement existed between phenotypic and genotypic features, there was generally a discrepancy. Strains with identical morphotypes show small differences in the 16S rRNA sequences, which might be related to the different chemical properties of their habitats. The results showed the importance of the polyphasic approach in order to improve the taxonomy of cyanobacteria.
Union Européenne = European Union - UE = EU
Researchers ; Professionals

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