No full text
Unpublished conference/Abstract (Scientific congresses and symposiums)
Biotechnological potential of the microflora associated with the brown alga Ascophyllum nodosum
Martin, Marjolaine; Martin, Renée; Barbeyron, Tristan et al.
2015Wolrd congress and expo on Applied Microbiology
 

Files


Full Text
No document available.
Full Text Parts
Biotechnological potential of novel bacterial strains isolated on.pdf
Author postprint (1.55 MB)
Request a copy
Marjolaine Martin AWARD.pdf
Author preprint (618.75 kB)
Best Young Researcher Award
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Algae; Microorganisms; polysaccharidase
Abstract :
[en] Bacteria associated with algae are underexplored despite their huge biodiversity and the fact that they differ markedly from those living freely in seawater. These bacterial communities are known to represent great potential for the production of diverse bioactive compounds, such as specific glycoside hydrolases, as they interact in multiple complex ways with their host. Furthermore, enzymes from marine bacteria have original properties, like cold-adapted, halotolerant and highly stable, which are constantly searched out by bio-industries. The aim of our study was to identify bacteria, associated with the brown alga Ascophyllum nodosum, showing diverse polysaccharolytic activities. To isolate cultivable microorganisms, algal thalli of Ascophyllum nodosum were swabbed with sterile cotton tips and marine agar plates were inoculated. Three-hundred isolated bacteria were screened for agarase, kappa- and iota-carrageenase, and sulfatase activities on specific marine media. Thirty-two bacteria with polysaccharolytic activities were isolated and a part of their 16S rDNA (8F-1492R) were amplified and sequenced. Twenty-seven were classified as Flavobacteriia and five as Gammaproteobacteria. Putative new strains and species of Zobellia, Maribacter, Cellulophaga, Shewanella, Glaciecola, Pseudoalteromonas and Colwellia were identified by phylogenetic analysis. All those genera are well-known to colonize algal surface but only some of them are famous to degrade algal polysaccharides (Zobellia, Maribacter, Cellulophaga, and Pseudoalteromonas). However, all those novel bacterial strains/species showed multiple and diverse enzymatic activities (agarase, iota-and kappa-carrageenase, cellulase, beta-glucosidase, sulfatase and/or amylase activities). Genomics libraries with their DNA were constructed in Escherichia coli and Bacillus subtilis and are screened to identify the genes coding for the observed enzymatic activities. Those novel glycoside hydrolases from unknown marine bacteria should have original and innovative properties with great biotechnological potential.
Disciplines :
Microbiology
Author, co-author :
Martin, Marjolaine ;  Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Martin, Renée ;  Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Barbeyron, Tristan;  UPMC- CNRS, Station Biologique de Roscoff > UMR8227 > Integrative Biology of Marine Models
Portetelle, Daniel ;  Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Michel, Gurvan;  CNRS- UMPC Station Biologique de Roscoff > UMR 8227 > Integrative Biology of Marine Models
Vandenbol, Micheline ;  Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Language :
English
Title :
Biotechnological potential of the microflora associated with the brown alga Ascophyllum nodosum
Publication date :
18 August 2015
Event name :
Wolrd congress and expo on Applied Microbiology
Event place :
Francfort, Germany
Event date :
18 au 20 aout 2015
By request :
Yes
Audience :
International
References of the abstract :
Journal of Microbial and Biochemical Technology(2015), 7(4), p.179.
Commentary :
BEST YOUNG RESEARCHER AWARD
Available on ORBi :
since 26 January 2015

Statistics


Number of views
268 (20 by ULiège)
Number of downloads
47 (5 by ULiège)

Bibliography


Similar publications



Contact ORBi