The phosphorylated pathway of serine biosynthesis is essential both for male gametophyte and embryo development and for root growth in Arabidopsis

Cascales - Miñana, Borja; Muñoz-Bertomeu, J.; Flores-Tornero, M.; Anoman, A. D.; Pertusa, J.; Alaiz, M.; Osorio, S.; Fernie, A. R.; Segura, J.; Ros, R.

doi:10.1105/tpc.113.112359

Article (Scientific journals)

The phosphorylated pathway of serine biosynthesis is essential both for male gametophyte and embryo development and for root growth in Arabidopsis

Cascales - Miñana, Borja; Muñoz-Bertomeu, J.; Flores-Tornero, M. et al.

2013 • In Plant Cell, 25 (6), p. 2084-2101

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https://hdl.handle.net/2268/176022

DOI
10.1105/tpc.113.112359

PubMed
23771893

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Keywords :

Arabidopsis protein; PSP1 protein, Arabidopsis; Arabidopsis; Amino Acids; Arabidopsis Proteins; Biosynthetic Pathways; Citric Acid Cycle; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Glycolysis; Green Fluorescent Proteins; Immunoblotting; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Mutation; Phosphoric Monoester Hydrolases; Phosphorylation; Plant Roots; Plants, Genetically Modified; Pollen; Reverse Transcriptase Polymerase Chain Reaction; Seeds; Serine

Abstract :

[en] This study characterizes the phosphorylated pathway of Ser biosynthesis (PPSB) in Arabidopsis thaliana by targeting phosphoserine phosphatase (PSP1), the last enzyme of the pathway. Lack of PSP1 activity delayed embryo development, leading to aborted embryos that could be classified as early curled cotyledons. The embryo-lethal phenotype of psp1 mutants could be complemented with PSP1 cDNA under the control of Pro35S (Pro35S:PSP1). However, this construct, which was poorly expressed in the anther tapetum, did not complement mutant fertility. Microspore development in psp1.1/psp1.1 Pro35S:PSP1 arrested at the polarized stage. The tapetum from these lines displayed delayed and irregular development. The expression of PSP1 in the tapetum at critical stages of microspore development suggests that PSP1 activity in this cell layer is essential in pollen development. In addition to embryo death and male sterility, conditional psp1 mutants displayed a shortroot phenotype, which was reverted in the presence of Ser. A metabolomic study demonstrated that the PPSB plays a crucial role in plant metabolism by affecting glycolysis, the tricarboxylic acid cycle, and the biosynthesis of amino acids. We provide evidence of the crucial role of the PPSB in embryo, pollen, and root development and suggest that this pathway is an important link connecting primary metabolism with development. © 2013 American Society of Plant Biologists. All rights reserved.

Disciplines :

Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Cascales - Miñana, Borja ; Departament de Biologia Vegetal, Facultat de Farmàcia, Universitat de València, Burjassot, Valencia, Spain

Muñoz-Bertomeu, J.; Departament de Biologia Vegetal, Universitat de València, 46100 Burjassot (Valencia), Spain, Instituto de Biología Molecular y Celular de Plantas, Departamento Biotecnología, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Cientificas, C/Ingeniero Fausto Elio, 46022 Valencia, Spain

Flores-Tornero, M.; Departament de Biologia Vegetal, Universitat de València, 46100 Burjassot (Valencia), Spain

Anoman, A. D.; Departament de Biologia Vegetal, Universitat de València, 46100 Burjassot (Valencia), Spain

Pertusa, J.; Departament de Biologia Funcional i Antropologia Física, Universitat de València, 46100 Valencia, Spain

Alaiz, M.; Grupo de Componentes Bioactivos y Funcionales de Productos Vegetales, Departamento de Fisiología y Tecnología de Productos Vegetales, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, 41012 Seville, Spain

Osorio, S.; Max Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany

Fernie, A. R.; Max Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany

Segura, J.; Departament de Biologia Vegetal, Universitat de València, 46100 Burjassot (Valencia), Spain

Ros, R.; Departament de Biologia Vegetal, Universitat de València, 46100 Burjassot (Valencia), Spain

Language :

English

Title :

The phosphorylated pathway of serine biosynthesis is essential both for male gametophyte and embryo development and for root growth in Arabidopsis

Publication date :

2013

Journal title :

Plant Cell

ISSN :

1040-4651

eISSN :

1532-298X

Publisher :

American Society of Plant Biologists, Rockville, United States - Maryland

Volume :

Issue :

Pages :

2084-2101

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 08 January 2015

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