Reference : The plastidial glyceraldehyde-3-phosphate dehydrogenase is critical for viable pollen...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/176019
The plastidial glyceraldehyde-3-phosphate dehydrogenase is critical for viable pollen development in Arabidopsis
English
Muñoz-Bertomeu, Jesús [Departament de Biologia Vegetal, Facultat de Farmácia, Universitat de València, 46100 Burjassot, Valencia, Spain]
Cascales - Miñana, Borja mailto [Departament de Biologia Vegetal, Facultat de Farmácia, Universitat de València, 46100 Burjassot, Valencia, Spain > > > >]
Irles-Segura, Asunción [Departament de Biologia Funcional i Antropologia Física, Facultat de Biologia, Universitat de València, 46100 Burjassot, Valencia, Spain]
Mateu, Isabel [Departament de Botánica, Facultat de Biologia, Universitat de València, Burjassot, Valencia 46100, Spain]
Nunes-Nesi, Adriano [Max Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany]
Fernie, Alisdair R. [Max Planck Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany]
Segura, Juan [Departament de Biologia Vegetal, Facultat de Farmácia, Universitat de València, 46100 Burjassot, Valencia, Spain]
Ros, Roc [Departament de Biologia Vegetal, Facultat de Farmácia, Universitat de València, 46100 Burjassot, Valencia, Spain]
2010
Plant Physiology
152
4
1830-1841
Yes (verified by ORBi)
0032-0889
[en] Arabidopsis ; Arabidopsis ; Glyceraldehyde-3-Phosphate Dehydrogenases ; Plastids ; Pollen ; Arabidopsis ; Arabidopsis thaliana
[en] Plant metabolism is highly coordinated with development. However, an understanding of the whole picture of metabolism and its interactions with plant development is scarce. In this work, we show that the deficiency in the plastidial glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPCp) leads to male sterility in Arabidopsis (Arabidopsis thaliana). Pollen from homozygous gapcp double mutant plants (gapcp1gapcp2) displayed shrunken and collapsed forms and were unable to germinate when cultured in vitro. The pollen alterations observed in gapcp1gapcp2 were attributed to a disorganized tapetum layer. Accordingly, the expression of several of the genes involved in tapetum development was down-regulated in gapcp1gapcp2. The fertility of gapcp1gapcp2 was rescued by transforming this mutant with a construct carrying the GAPCp1 cDNA under the control of its native promoter (pGAPCp1::GAPCp1c). However, the GAPCp1 or GAPCp2 cDNA under the control of the 35S promoter (p35S::GAPCp), which is poorly expressed in the tapetum, did not complement the mutant fertility. Mutant GAPCp isoforms deficient in the catalytic activity of the enzyme were unable to complement the sterile phenotype of gapcp1gapcp2, thus confirming that both the expression and catalytic activity of GAPCp in anthers are necessary for mature pollen development. A metabolomic study in flower buds indicated that the most important difference between the sterile (gapcp1gapcp2, gapcp1gapcp2-p35S::GAPCp) and the fertile (wild-type plants, gapcp1gapcp2-pGAPCp1::GAPCp1c) lines was the increase in the signaling molecule trehalose. This work corroborates the importance of plastidial glycolysis in plant metabolism and provides evidence for the crucial role of GAPCps in pollen development. It additionally brings new insights into the complex interactions between metabolism and development. © 2010 American Society of Plant Biologists.
http://hdl.handle.net/2268/176019
10.1104/pp.109.150458

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