Article (Scientific journals)
Plastidial glyceraldehyde-3-phosphate dehydrogenase deficiency leads to altered root development and affects the sugar and amino acid balance in Arabidopsis
Muñoz-Bertomeu, Jesús; Cascales - Miñana, Borja; Mulet, Juan M. et al.
2009In Plant Physiology, 151 (2), p. 541-558
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Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Deficiency Leads to Altered Root Development and Affects the Sugar and Amino Acid Balance in Arabidopsis.pdf
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Keywords :
Arabidopsis protein; Arabidopsis; Amino Acids; Arabidopsis Proteins; Carbohydrate Metabolism; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes, Plant; Genetic Complementation Test; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); Glycolysis; Lipid Metabolism; Mutation; Phenotype; Phylogeny; Plant Leaves; Plant Roots; Plastids; Protein Transport; RNA, Messenger; Serine; Arabidopsis thaliana
Abstract :
[en] Glycolysis is a central metabolic pathway that, in plants, occurs in both the cytosol and the plastids. The glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate with concomitant reduction of NAD+ to NADH. Both cytosolic (GAPCs)and plastidial(GAPCps)GAPDH activities have been described. However, the in vivo functions of the plastidial isoforms remain unresolved. In this work, we have identified two Arabidopsis (Arabidopsis thaliana) chloroplast/plastid-localized GAPDH isoforms (GAPCp1 and GAPCp2). gapcp double mutants display a drastic phenotype of arrested root development, dwarfism,and sterility. In spite of their low gene expression level as compared with other GAPDHs, GAPCp down-regulation leads to altered gene expression and to drastic changes in the sugar and amino acid balance of the plant. We demonstrate that GAPCps are important for the synthesis of serine in roots. Serine supplementation to the growth medium rescues root developmental arrest and restores normal levels of carbohydrates and sugar biosynthetic activities in gapcp double mutants. We provide evidence that the phosphorylated pathway of Ser biosynthesis plays an important role in supplying serine to roots. Overall, these studies provide insights into the in vivo functions of the GAPCps in plants. Our results emphasize the importance of the plastidial glycolytic pathway, and specifically of GAPCps, in plant primary metabolism. © 2009 American Society of Plant Biologists.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Muñoz-Bertomeu, Jesús;  Departament de Biologia Vegetal, Facultat de Farmàcia, Universitat de València, 46100 Burjassot, Valencia, Spain
Cascales - Miñana, Borja ;  Departament de Biologia Vegetal, Facultat de Farmácia, Universitat de València, 46100 Burjassot, Valencia, Spain
Mulet, Juan M.;  Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain
Baroja-Fernández, Edurne;  Instituto de Agrobiotecnología, Consejo Superior de Investigaciones Científicas, Universidad Pública de Navarra, Gobierno de Navarra, 31192 Mutiloabeti, Nafarroa, Spain
Pozueta-Romero, Javier;  Instituto de Agrobiotecnología, Consejo Superior de Investigaciones Científicas, Universidad Pública de Navarra, Gobierno de Navarra, 31192 Mutiloabeti, Nafarroa, Spain
Kuhn, Josef M.;  Division of Biological Sciences, Cell and Developmental Biology Section, University of California San Diego, La Jolla, CA 92093-0116, United States, BASF Plant Science Company GmbH, Carl-Bosch-Strasse 64, 67117 Limburgerhof, Germany
Segura, Juan;  Departament de Biologia Vegetal, Facultat de Farmàcia, Universitat de València, 46100 Burjassot, Valencia, Spain
Ros, Roc;  Departament de Biologia Vegetal, Facultat de Farmàcia, Universitat de València, 46100 Burjassot, Valencia, Spain
Language :
English
Title :
Plastidial glyceraldehyde-3-phosphate dehydrogenase deficiency leads to altered root development and affects the sugar and amino acid balance in Arabidopsis
Publication date :
2009
Journal title :
Plant Physiology
ISSN :
0032-0889
eISSN :
1532-2548
Publisher :
American Society of Plant Biologists, United States - Maryland
Volume :
151
Issue :
2
Pages :
541-558
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 08 January 2015

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