Reference : A lateral belt of cortical LGN and NuMA guides mitotic spindle movements and planar d...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/175730
A lateral belt of cortical LGN and NuMA guides mitotic spindle movements and planar division in neuroepithelial cells
English
Peyre, Elise mailto [Université de Liège - ULiège > > Giga - Neurosciences >]
Jaouen, Florence [Université de la Méditerranée/Aix-Marseille II > Developmental Biology Institute (IBDML) > CNRS UMR6216 > >]
Saadaoui, Mehdi [Université de la Méditerranée/Aix-Marseille II > Developmental Biology Institute (IBDML) > CNRS UMR6216 > >]
Haren, Laurence [CNRS-Pierre Fabre > > > >]
Merdes, Andreas [CNRS-Pierre Fabre > > > >]
Durbec, Pascale [Université de la Méditerranée/Aix-Marseille II, > Developmental Biology Institute (IBDML) > CNRS UMR6216 > >]
Morin, Xavier [Ecole Normale Supérieure, Institut de Biologie de l’ENS, IBENS > INSERM, U1024; CNRS, UMR 8197 > > >]
28-Mar-2011
Journal of Cell Biology
Rockefeller University Press
Yes (verified by ORBi)
International
0021-9525
1540-8140
New York
NY
[en] To maintain tissue architecture, epithelial cells divide in a planar fashion, perpendicular to their main polarity axis. As the centrosome resumes an apical localization in interphase, planar spindle orientation is reset at each cell cycle. We used three-dimensional live imaging of GFP-labeled centrosomes to investigate the dynamics of spindle orientation in chick neuroepithelial cells. The mitotic spindle displays stereotypic movements during metaphase, with an active phase of planar orientation and a subsequent phase of planar maintenance before anaphase. We describe the localization of the NuMA and LGN proteins in a belt at the lateral cell cortex during spindle orientation. Finally, we show that the complex formed of LGN, NuMA, and of cortically located Gai subunits is necessary for spindle movements and regulates the dynamics of spindle orientation. The restricted localization of LGN and NuMA in the lateral belt is instructive for the planar alignment of the mitotic spindle, and required for its planar maintenance
http://hdl.handle.net/2268/175730
10.1083/jcb.201101039

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