[en] The aim of this retrospective study was to compare the number of follicles, cumulus oocyte complexes (COCs) and cultured In Vitro Produced (IVP) embryos obtained from 1396 non-stimulated Ovum Pick-up (OPU) sessions on 81 donor animals in a twice weekly OPU scheme. Results were obtained from 640 sessions following FSH-LH superstimulation, on 112 donors subjected to OPU once every 2 weeks. The stimulation protocol started with the insertion of an ear implant containing 3 mg norgestomet (Crestar, Intervet, Belgium) 8 days before puncture (day -8). The dominant follicle was ablated by ultrasound-guided follicle puncture on day -6. On day -3 and day -2, cows were injected with FSH (Ovagen, ICP) twice daily (8 am to 8 pm), i.e. a total dose of 160 mug FSH and 40 mug LG per donor per stimulation cycle. Animals were punctured 48 h after the last FSH injection (day 0). Progesterone implants were removed the next day. Stimulated donor cows were treated with this protocol at 14-day intervals. Follicles were visualized with a Dynamic Imaging ultrasound scanner, equipped with a 6.5 MHz sectorial probe. Follicles were punctured with 55 cm long, 18 gauge needles at an aspiration pressure corresponding to a flow rate of 15 ml/min. Cumulus oocyte complexes were recovered and processed in a routine IVF set-up. Results demonstrate that, expressed per session, FSH stimulation prior to OPU increases production efficiency with significantly more follicles punctured and oocytes retrieved. However, when overall results during comparable 2-week periods are considered (four non-stimulated sessions vs one stimulated), more follicles are punctured and more oocytes are retrieved using the non-stimulated protocol. No significant differences in the number of cultured embryos could be detected, indicating that FSH/LH stimulation prior to OPU might have a positive effect on in vitro oocyte developmental competence as more embryos are cultured with less, presumably better-quality, oocytes
Disciplines :
Veterinary medicine & animal health Biotechnology
Author, co-author :
De Roover, Rudolf
Feugang, J. M.
Bols, P. E. J.
Genicot, Gaëtan
Hanzen, Christian ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Obstétr. et path. de la reprod. des équidés, rumin. et porcs
Language :
English
Title :
Effects of ovum pick-up frequency and FSH stimulation: a retrospective study on seven years of beef cattle in vitro embryo production.
Blondin P, Sirard MA, 1995 : Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes. Mol Reprod Dev 41, 54 62.
Bols PEJ, Van Soom A, Ysebaert MT, Vandenheede JMM, de Kruif A, 1996 : Effects of aspiration vacuum and needle diameter on cumulus oocyte complex morphology and developmental capacity of bovine oocytes. Theriogenology 45, 1001 1014.
Bols PEJ, Leroy JLMR, Vanholder T, Van Soom A, 2004 : A comparison of a mechanical sector and a linear array transducer for ultrasound-guided transvaginal oocyte retrieval (OPU) in the cow. Theriogenology 62, 906 914.
Bousquet D, Milovanov C, Bell JC, Durocher J, Smith LC, 1995 : Nuclear and cytoplasmic maturation of oocytes aspirated from large follicles in superovulated heifers. Theriogenology 43, 172.
Cushman RA, DeSouza JC, Hedgpeth VS, Britt JH, 1999 : Superovulatory response of one ovary is related to the micro- and macroscopic population of follicles in the contralateral ovary of the cow. Biol Reprod 60, 349 354.
De Roover R, Genicot G, Leonard S, Bols P, Dessy F, 2005 : Ovum pick up and in vitro embryo production in cows superstimulated with an individually adapted superstimulation protocol. Anim Reprod Sci 86, 13 25.
Ferry L, Mermillod P, Massip A, Dessy F, 1994 : Bovine embryos cultured in serum-poor oviduct-conditioned medium need cooperation to reach the blastocyst stage. Theriogenology 42, 445 453.
Galli C, Crotti G, Notari C, Turini P, Duchi R, Lazzari G, 2001 : Embryoproduction by ovum pick-up from live donors. Theriogenology 55, 1341 1357.
Goodhand KL, Staines ME, Hutchinson JSM, Broadbent PJ, 2000 : In vivo oocyte recovery and in vitro embryo production from bovine oocyte donors treated with progestagen, oestradiol and FSH. Anim Reprod Sci 63, 145 158.
Hashimoto S, Takakura R, Minami N, Yamada M, 1999 : Ultrasound-guided follicle aspiration: effect of the frequency of a linear transvaginal probe on the collection of bovine oocytes. Theriogenology 52, 131 138.
Hasler JF, 1998 : The current status of oocyte recovery, in vitro embryo production, and embryo transfer in domestic animals, with an emphasis on the bovine. J Anim Sci 76 (Suppl. 3 52 74.
Kruip ThAM, Boni R, Wurth YA, Roelofsen MWM, Pieterse MC, 1994 : Potential use of ovum pick-up for embryo production and breeding in cattle. Theriogenology 42, 675 684.
Lerner SP, Thayne WV, Baker RD, Hensche T, Meredith S, Inskeep EK, Dailey RA, Lewis PE, Butcher RL, 1986 : Age, dose of FSH and other factors affecting superovulation in Holstein cows. J Anim Sci 63, 176 183.
Lonergan P, Monaghan P, Rizos D, Boland MP, Gordon I, 1994 : Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization and culture in vitro. Mol Reprod Dev 37, 48 53.
Looney CR, Lindsey BR, Gonseth CL, Johnson DL, 1994 : Commercial aspects of oocyte retrieval and in vitro fertilization (IVF) for embryo production in problem cows. Theriogenology 41, 67 72.
de Loos FAM, Bevers MM, Dieleman SJ, Kruip ThAM, 1991 : Morphology of preovulatory bovine follicles as related to oocyte maturation. Theriogenology 35, 527 535.
Lu KH, Shi DS, Jiang HS, Goulding D, Boland MP, Roche JF, 1991 : Comparison of the developmental capacity of bovine oocytes from superovulated and non stimulated heifers. Theriogenology 35, 234.
Machatkova M, Krausova K, Jokesova E, Tomanek M, 2004 : Developmental competence of bovine oocytes: effects of follicle size and the phase of follicular wave on in vitro embryo production. Theriogenology 61, 329 335.
Meintjens M, Bellow MS, Broussard JR, Paul JB, Godke RA, 1995 : Transvaginal aspiration of oocytes from hormone-treated pregnant beef cattle for in vitro fertilization. J Anim Sci 73, 967 974.
Merton JS, de Roos APW, Mullaart E, de Ruigh L, Kaal L, Vos PLAM, Dieleman SJ, 2003 : Factors affecting oocyte quality and quantity in commercial application of embryo technologies in the cattle breeding industry. Theriogenology 59, 651 674.
Paul JB, Looney CR, Lindsay BR, Godke RA, 1995 : Gonadotropin stimulation of cattle donors at estrus for transvaginal oocyte collection. Theriogenology 43, 294.
Pieterse MC, Kappen KA, Kruip ThAM, Taverne MAM, 1988 : Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries. Theriogenology 30, 751 762.
Pieterse MC, Vos PLAM, Kruip ThAM, Wurth YA, Van Beneden ThH, Willemse AH, Taverne MAM, 1991 : Transvaginal ultrasound guided follicular aspiration of bovine oocytes. Theriogenology 35, 857 862.
Pieterse MC, Vos PLAM, Kruip ThAM, Wurth YA, Van Beneden ThH, Willemse AH, Taverne MAM, 1992 : Repeated transvaginal ultrasound-guided ovum pick-up in ECG-treated cows. Theriogenology 37, 273.
Ptak G, Tischner M, Bernabò N, Loi P, 2003 : Donor-dependent developmental competence of oocytes from lambs subjected to repeated hormonal stimulation. Biol Reprod 69, 278 285.
Seneda MM, Esper CR, Garcia JM, Andrade ER, Binelli M, Oliveira JA, Nascimento AB, 2003 : Efficacy of linear and convex transducers for ultrasound-guided transvaginal follicle aspiration. Theriogenology 59, 1435 1440.
Sirard MA, Picard L, Dery M, Coenen K, Blondin P, 1999 : The time interval between FSH administration and ovarian aspiration influences the development of cattle oocytes. Theriogenology 51, 699 708.
Stubbings RB, Walton JS, 1995 : Effect of ultrasonically-guided follicle aspiration on estrous cycle and follicular dynamics in Holstein cows. Theriogenology 43, 705 712.
Taneja M, Bols PEJ, Van de Velde A, Ju JC, Schreiber D, Tripp MW, Levine H, Echelard Y, Riesen J, Yang X, 2000 : Developmental competence of juvenile calf oocytes in vitro and in vivo: influence of donor animal variation and repeated gonadotropin stimulation. Biol Reprod 62, 206 213.
Van Soom A, Bols PEJ, de Kruif A, 1995 : Improved results in IVF-treatment of sterility patients by careful selection of bulls and by stimulation of cows with FSH before slaughter (28. TAGUNG PHYSIOLOGIE UND PATHOLOGIE DER FORTPFLANZUNG). Reprod Dom Anim 29 (Suppl. 3 1 16.
Walton JS, Christie KA, Stubbings RB, 1993 : Evaluation of frequency of ultrasonically guided follicle aspiration on bovine ovarian dynamics. Theriogenology 39, 336.
Ward FA, Lonergan P, Enright BP, Boland MP, 2000 : Factors affecting recovery and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology. Theriogenology 54, 433 446.