Effects of ovum pick-up frequency and FSH stimulation: a retrospective study on seven years of beef cattle in vitro embryo production.

De Roover, Rudolf; Feugang, J. M.; Bols, P. E. J.; Genicot, Gaëtan; Hanzen, Christian

doi:10.1111/j.1439-0531.2007.00873.x

Article (Scientific journals)

Effects of ovum pick-up frequency and FSH stimulation: a retrospective study on seven years of beef cattle in vitro embryo production.

De Roover, Rudolf; Feugang, J. M.; Bols, P. E. J. et al.

2008 • In Reproduction in Domestic Animals, 43 (2), p. 239-245

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https://hdl.handle.net/2268/175

DOI
10.1111/j.1439-0531.2007.00873.x

PubMed
18226024

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Keywords :

ovum pick-up; embryo; cattle; embryon; bovin

Abstract :

[en] The aim of this retrospective study was to compare the number of follicles, cumulus oocyte complexes (COCs) and cultured In Vitro Produced (IVP) embryos obtained from 1396 non-stimulated Ovum Pick-up (OPU) sessions on 81 donor animals in a twice weekly OPU scheme. Results were obtained from 640 sessions following FSH-LH superstimulation, on 112 donors subjected to OPU once every 2 weeks. The stimulation protocol started with the insertion of an ear implant containing 3 mg norgestomet (Crestar, Intervet, Belgium) 8 days before puncture (day -8). The dominant follicle was ablated by ultrasound-guided follicle puncture on day -6. On day -3 and day -2, cows were injected with FSH (Ovagen, ICP) twice daily (8 am to 8 pm), i.e. a total dose of 160 mug FSH and 40 mug LG per donor per stimulation cycle. Animals were punctured 48 h after the last FSH injection (day 0). Progesterone implants were removed the next day. Stimulated donor cows were treated with this protocol at 14-day intervals. Follicles were visualized with a Dynamic Imaging ultrasound scanner, equipped with a 6.5 MHz sectorial probe. Follicles were punctured with 55 cm long, 18 gauge needles at an aspiration pressure corresponding to a flow rate of 15 ml/min. Cumulus oocyte complexes were recovered and processed in a routine IVF set-up. Results demonstrate that, expressed per session, FSH stimulation prior to OPU increases production efficiency with significantly more follicles punctured and oocytes retrieved. However, when overall results during comparable 2-week periods are considered (four non-stimulated sessions vs one stimulated), more follicles are punctured and more oocytes are retrieved using the non-stimulated protocol. No significant differences in the number of cultured embryos could be detected, indicating that FSH/LH stimulation prior to OPU might have a positive effect on in vitro oocyte developmental competence as more embryos are cultured with less, presumably better-quality, oocytes

Disciplines :

Veterinary medicine & animal health
Biotechnology

Author, co-author :

De Roover, Rudolf

Feugang, J. M.

Bols, P. E. J.

Genicot, Gaëtan

Hanzen, Christian ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Obstétr. et path. de la reprod. des équidés, rumin. et porcs

Language :

English

Title :

Effects of ovum pick-up frequency and FSH stimulation: a retrospective study on seven years of beef cattle in vitro embryo production.

Publication date :

2008

Journal title :

Reproduction in Domestic Animals

ISSN :

0936-6768

eISSN :

1439-0531

Publisher :

Blackwell Publishing, Berlin, Germany

Volume :

Issue :

Pages :

239-245

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 03 July 2008

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