[en] A method for the determination of seven polychlorinated biphenyl residues in plasma by gas chromatography-mass spectrometry was developed. The analytes were isolated from human plasma by liquid-liquid extraction, followed by solid-phase extraction, and separation on an HP5 Trace column. Ionization mode was electronic impact, and selected ion storage was used for isolation and quantitation of the compounds. The method was evaluated for its analytical performances and therefore applied to monitor the prevalence of these seven polychlorinated biphenyl residues in a female population. Eighty samples were analyzed, and 82.5% presented detectable amounts of at least one residue. For the population study, results were reported on a lipid-adjusted basis. Because the proposed method is satisfying and seems to be suitable for the quantitation of PCB congeners in a general population, epidemiological studies to evaluate the widespread contamination with these environmental compounds can be proposed.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Charlier, Corinne ; Université de Liège - ULiège > Département de pharmacie > Chimie toxicologique
Dubois, Nathalie ; Centre Hospitalier Universitaire de Liège - CHU > Toxicologie clinique
CUCCHIARO, Séverine ; Centre Hospitalier Universitaire de Liège - CHU > Service médical de radiothérapie
Plomteux, Guy ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Analysis of polychlorinated biphenyl residues in human plasma by gas chromatography-mass spectrometry
A. Pauwels, A. Cabei, J. Weyler, L. Delbeke, M. Dhont, P. De Sutter, T. D'Hooghe, and P. Schepens. Comparison of persistent organic pollutants residues in serum and adipose tissue in a female population in Belgium, 1996-1998. Arch. Environ. Contam. Toxicol. 39: 265-270 (2000).
L. Asplund, B. Svensson, U. Eriksson, B. Jansson, S. Jensen, U. Widequist, and S. Skerfuing. PCBs, DDT, DDE in human plasma related to fish consumption. Arch. Environ. Health 49: 477-486 (1994).
H. Bradlow, D. Davis, G. Lin, D. Sepkovic, and R. Tiwari. Effects of pesticides on the ratio of 16a/2 hydroxyestrone: a biologic marker of breast cancer risk. Environ. Health Perspect. 103: 147-150 (1995).
P. Siiteri. Adipose tissues as a source of hormones. Am. J. Clin. Nutr. 45: 277-282 (1987).
M. Reickman, J. Iudd, and C. Longcape. Effect of alcohol consumption on plasma and urinary hormone concentrations in premenopausal women. J. Natl. Cancer Inst. 85: 722-727 (1993).
S. Arnold, D. Klotz, B. Collins, P. Vanier, L. Guillette, and J. McLachlan. Synergistic activation of estrogen receptor with combinations of environmental chemicals. Sciences 272: 1489-1492 (1996).
P. Kodavanti, T. Ward, E. Derr-Yellin, W. Mundy, A. Casey, B. Bush, and H. Tilson. Congener-specific distribution of PCBs in brain regions, blood, liver, and fat of adult rats following repeated exposure to Arochlor 1254. Toxicol. Appl. Pharmacol. 153: 199-210 (1998).
P. Sohoni and J. Sumpter. Several environmental estrogen are also anti-androgens. J. Endocrinol. 158: 327-339 (1998).
A. Demers, P. Ayotte, J. Brisson, S. Dodin, J. Robert, and E. Dewailly. Risk and aggressiveness of breast cancer in relation to plasma organochlorine concentrations. Cancer Epidemiol. Biomarkers Prev. 9: 161-166 (2000).
L. Fleming, J. Mann, J. Bean, T. Briggle, and J. Sanchez-Ramos. Parkinson's disease and brain levels of organochlorine pesticides. Ann. Neurol. 36: 100-103 (1994).
C. Charlier and G. Plomteux. Determination of organochlorines pesticides in blood of healthy individuals. Clin. Chem. Lab. Med. 40: 361-364 (2002).
D. Phillips, J. Pirkle, V. Burse, J. Bernert, L. Henderson, and L. Needham. Chlorinated hydrocarbon levels in human serum: effects of fasting and feeding. Arch. Environ. Contam. Toxicol. 18: 495-500 (1989).
M. Dressler. Selective gas chromatographic detectors. J. Chromatogr. 36: (1986).
K. Ballschmiter, R. Bacher, A. Mennel, R. Fischer, U. Riehle, and M. Smerev. The determination of chlorinated biphenyls, chlorinated dibenzodioxins and chlorinated dibenzofurans by GC-MS. J. High Res. Chromatogr. 15: 260-267 (1992).