Relationship of progesterone, bovine pregnancy-associated glycoprotein-1 and nitric oxide with late embryonic and early fetal mortalities in dairy cows
Karen, A; Bajcsy, AC; Minoia, Ret al.
2014 • In Journal of Reproduction and Development, 60 (2), p. 162-167
[en] The aim of the present study was to determine the relationship of progesterone (P4), bovine pregnancy-associated glycoprotein-1 (bPAG-1) and nitric oxide (NO) levels with late embryonic (LEM; day 28 to day 42) and early fetal mortalities (EFM; > day 42 to day 56) in dairy cows. Transrectal ultrasonography (6-8 MHz) was performed in 100 Holstein-Friesian cows at days 28, 42 and 56 after artificial insemination (AI; day 0) to diagnose pregnancy and to monitor the fate of the embryo. After ultrasound scanning of each cow, a milk sample was collected for assessment of P4 by an ELISA test and a blood sample was collected for assessment of bPAG-1, by using a double-antibody radioimmunoassay, and serum NO metabolites (nitrate + nitrite). Based on ultrasonographic examinations and bPAG-1-RIA, 41 of 100 inseminated cows were confirmed pregnant at day 28 after AI. Nine cows suffered of LEM, and 6 cows suffered of EFM and the overall pregnancy loss rate was 36.6% (15/41) between days 28 and 56 of pregnancy. By logistic regression analysis, there were no significant relationships between the level of P4 and bPAG-1 at day 28 after AI and the occurrence of LEM and EFM. Also, there were no significant relationships between the levels of P4 and bPAG-1 at day 42 and the occurrence of EFM. On the other hand, a significant relationship (P<0.05) was found between NO level at day 28 and the occurrence of LEM. In conclusion, measurement of the serum NO concentration at day 28 of pregnancy might help to predict the outcome of pregnancy by day 42 in dairy cows but further studies are needed to confirm this.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Karen, A
Bajcsy, AC
Minoia, R
Kovacs, R
Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles (DSF) > Physiologie de la reproduction
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles (DSF) > Département de sciences fonctionnelles (DSF)
Tibold, J
Madl, I
Szenci, O
Language :
English
Title :
Relationship of progesterone, bovine pregnancy-associated glycoprotein-1 and nitric oxide with late embryonic and early fetal mortalities in dairy cows
De Vries A. Economic value of pregnancy in dairy cattle. J Dairy Sci 2006; 89: 3876-3885. [Medline] [CrossRef]
Whitlock BK, Maxwell HS. Pregnancy-associated glycoproteins and pregnancy wastage in cattle. Theriogenology 2008; 70: 550-559. [Medline] [CrossRef]
López-Gatius F. Factors of a noninfectious nature affecting fertility after artificial insemination in lactating dairy cows. A review. Theriogenology 2012; 77: 1029-1041. [Medline] [CrossRef]
Starbuck MJ, Dailey RA, Inskeep EK. Factors affecting retention of early pregnancy in dairy cattle. Anim Reprod Sci 2004; 84: 27-39. [Medline] [CrossRef]
López-Gatius F, Hunter RH, Garbayo JM, Santolaria P, Yániz J, Serrano B, Ayad A, de Sousa NM, Beckers JF. Plasma concentrations of pregnancy-associated glycoprotein-1 (PAG-1) in high producing dairy cows suffering early fetal loss during the warm season. Theriogenology 2007; 67: 1324-1330. [Medline] [CrossRef]
Humblot F, Camous S, Martal J, Charlery J, Jeanguyot N, Thibier M, Sasser RG. Pregnancy-specific protein B, progesterone concentrations and embryonic mortality during early pregnancy in dairy cows. J Reprod Fertil 1988; 83: 215-223. [Medline] [Cross-Ref]
Bech-Sàbat G, López-Gatius F, Yániz JL, García-Ispierto I, Santolaria P, Serrano B, Sulon J, de Sousa NM, Beckers JF. Factors affecting plasma progesterone in the early fetal period in high producing dairy cows. Theriogenology 2008; 69: 426-432. [Medline] [CrossRef]
Wooding FB, Roberts RM, Green JA. Light and electron microscope immunocytochemical studies of the distribution of pregnancy associated glycoproteins (PAGs) throughout pregnancy in the cow: possible functional implications. Placenta 2005; 26: 807-827. [Medline] [CrossRef]
Zoli AP, Guilbault LA, Delahaut P, Ortiz WB, Beckers JF. Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biol Reprod 1992; 46: 83-92. [Medline] [CrossRef]
Breukelman SP, Perényi Z, Taverne MAM, Jonker H, van der Weijden GC, Vos PLAM, de Ruigh L, Dieleman SJ, Beckers JF, Szenci O. Characterisation of pregnancylosses after embryo transfer by measuring plasma progesterone and bovine pregnancyassociated glycoprotein-1 concentrations. Vet J 2012; 194: 71-76. [Medline] [CrossRef]
Semambo DK, Eckersall PD, Sasser RG, Ayliffe TR. Pregnancy-specific protein B and progesterone in monitoring viability of the embryo in early pregnancy in the cow after experimental infection with Actinomyces pyogenes. Theriogenology 1992; 37: 741-748. [Medline] [CrossRef]
Szenci O, Humblot P, Beckers JF, Sasser G, Sulon J, Baltusen R, Varga J, Bajcsy CA, Taverne MA. Plasma profiles of progesterone and conceptus proteins in cows with spontaneous embryonic/fetal mortality as diagnosed by ultrasonography. Vet J 2000; 159: 287-290. [Medline] [CrossRef]
Szenci O, Beckers JF, Sulon J, Bevers MM, Börzsönyi L, Fodor L, Kovács F, Taverne MAM. Effect of induction of late embryonic mortality on plasma profiles of pregnancy associated glycoprotein 1 in heifers. Vet J 2003; 165: 307-313. [Medline] [CrossRef]
Gábor G, Tóth F, Ozsvári L, Abonyi-Tóth Z, Sasser RG. Early detection of pregnancy and embryonic loss in dairy cattle by ELISA tests. Reprod Domest Anim 2007; 42: 633-636. [Medline] [CrossRef]
Knowles RG, Moncada S. Nitric oxide synthases in mammals. Biochem J 1994; 298: 249-258. [Medline]
Korzekwa A, Woclawek-Potocka I, Okuda K, Acosta TJ, Skarzynski DJ. Nitric oxides in bovine corpus luteum: possible mechanisms of action in luteolysis. Anim Sci J 2007; 78: 233-242. [CrossRef]
Groebner AE, Schulke KS, Bauersachs S, Wolf E, Meyer HHD, Ulbrich SE. Expression of inducible and endothelial nitric oxide synthase in the bovine endometrium during estrouscycle and early pregnancy. Reprod Domest Anim 2008; 43(Suppl.1): 12. [Medline]
Soto P, Natzke RP, Hansen PJ. Identification of possible mediators of embryonic mortality caused by mastitis: actions of lipopolysaccharide, prostaglandin F2α, and the nitric oxide generator, sodium nitroprusside dihydrate, on oocyte maturation and embryonic development in cattle. Am J Reprod Immunol 2003; 50: 263-272. [Medline] [CrossRef]
Bird IM, Zhang L, Magness RR. Possible mechanisms underlying pregnancy-induced changes in uterine artery endothelial function. Am J Physiol Regul Integr Comp Physiol 2003; 284: R245-R258. [Medline]
Ferguson JD, Galligan DT, Thomsen N. Principal descriptors of body condition score in Holstein cows. J Dairy Sci 1994; 77: 2695-2703. [Medline] [CrossRef]
Szenci O, Beckers JF, Humblot P, Sulon J, Sasser G, Taverne MAM, Varga J, Baltusen R, Schekk G. Comparison of ultrasonography, bovine pregnancy-specific protein B, and bovine pregnancy-associated glycoprotein 1 tests for pregnancy detection in dairy cows. Theriogenology 1998; 50: 77-88. [Medline] [CrossRef]
López-Gatius F, Santolaria P, Yániz J, Rutllant J, López-Béjar M. Factors affecting pregnancy loss from gestation Day 38 to 90 in lactating dairy cows from a single herd. Theriogenology 2002; 57: 1251-1261. [Medline] [CrossRef]
Perényi ZS, Szenci O, Sulon J, Drion PV, Beckers JF. Comparison of the ability of three radioimmunoassay to detect pregnancy-associated glycoproteins in bovine plasma. Reprod Domest Anim 2002; 37: 100-104. [Medline] [CrossRef]
Perényi ZS, Szenci O, Drion PV, Banga-Mboko H, Sousa NM, El Amiri B, Beckers JF. Aspartic proteinase members secreted by the ruminant placenta: specificity of three radioimmunoassay systems for the measurement of pregnancy-associated glycoproteins. Reprod Domest Anim 2002; 37: 324-329. [Medline] [CrossRef]
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem 1982; 126: 131-138. [Medline] [CrossRef]
Hosmer DW, Lemeshow S. Applied Logistic Regression, John Wiley & Sons, New York; 1989.
Fricke PM, Guenther JN, Wiltbank MC. Efficacy of decreasing the dose of GnRH used in a protocol for synchronization of ovulation and timed AI in lactating dairy cows. Theriogenology 1998; 50: 1275-1284. [Medline] [CrossRef]
Cartmill JA, El-Zarkouny SZ, Hensley BA, Lamb GC, Stevenson JS. Stage of cycle, incidence, and timing of ovulation, and pregnancy rates in dairy cattle after three timed breeding protocols. J Dairy Sci 2001; 84: 1051-1059. [Medline] [CrossRef]
Cartmill JA, El-Zarkouny SZ, Hensley BA, Rozell TG, Smith JF, Stevenson JS. An alternative AI breeding protocol for dairy cows exposed to elevated ambient temperatures before or after calving or both. J Dairy Sci 2001; 84: 799-806. [Medline] [CrossRef]
Gümen A, Guenther JN, Wiltbank MC. Follicular size and response to Ovsynch versus detection of estrus in anovular and ovular lactating dairy cows. J Dairy Sci 2003; 86: 3184-3194. [Medline] [CrossRef]
Sartori R, Gümen A, Guenther JN, Souza AH, Caraviello DZ, Wiltbank MC. Comparison of artificial insemination versus embryo transfer in lactating dairy cows. Theriogenology 2006; 65: 1311-1321. [Medline] [CrossRef]
Gábor G, Tóth F, Ozsvári L, Abonyi-Tóth Z, Sasser R. Factors influencing pregnancy rate and late embryonic loss in dairy cattle. Reprod Domest Anim 2008; 43: 53-58. [Medline]
Bulman DC, Lamming GE. Milk progesterone levels in relation to conception, repeat breeding and factors influencing acyclicity in dairy cows. J Reprod Fertil 1978; 54: 447-458. [Medline] [CrossRef]
Cox NM, Thompson FN, Culver DH. Milk progesterone to predict reproductive status in a commercial dairy herd. J Dairy Sci 1978; 61: 1616-1621. [Medline] [CrossRef]
Hayashi KG, Hosoe M, Takahashi T. Placental expression and localization of endothelin-1 system and nitric oxide synthases during bovine pregnancy. Anim Reprod Sci 2012; 134: 150-157. [Medline] [CrossRef]
Vonnahme KA, Wilson ME, Li Y, Rupnow HL, Phernetton TM, Ford SP, Magness RR. Circulating levels of nitric oxide and vascular endothelial growth factor throughout ovine pregnancy. J Physiol 2005; 565: 101-109. [Medline] [CrossRef]
Kaufmann TB, Drillich M, Tenhagen BA, Forderung D, Heuwieser W. Prevalence of bovine subclinical endometritis 4h after insemination and its effects on first service conception rate. Theriogenology 2009; 71: 385-391. [Medline] [CrossRef]
Li D, Liu Y, Li Y, Lv Y, Pei X, Guo D. Significance of nitric oxide concentration in plasma and uterine secretes with puerperal endometritis in dairy cows. Vet Res Commun 2010; 34: 315-321. [Medline] [CrossRef]
Aytekin I, Aypak SU. Levels of selected minerals, nitric oxide, and vitamins in aborted Sakis sheep raised under semitropical conditions. Trop Anim Health Prod 2011; 43: 511-514. [Medline] [CrossRef]
Gilbert RO. The effects of endometritis on the establishment of pregnancy in cattle. Reprod Fertil Dev 2011; 24: 252-257. [Medline] [CrossRef]
