Article (Scientific journals)
QTL analysis and candidate gene mapping for the polyphenol content in cider apple
Verdu, Cindy; Guyot, Sylvain; Childebrand, Nicolas et al.
2014In PLoS ONE, 9 (10), p. 107103
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Keywords :
candidate gene identification; cider apples; Malus x domestica; mean polymerization degree; phenolic compounds; polyphenol biosynthesis; Quantitative Trait Loci
Abstract :
[en] Polyphenols have favorable antioxidant potential on human health suggesting that their high content is responsible for the beneficial effects of apple consumption. They control the quality of ciders as they predominantly account for astringency, bitterness, color and aroma. In this study, we identified QTLs controlling phenolic compound concentrations and the average polymerization degree of flavanols in a cider apple progeny. Thirty two compounds belonging to five groups of phenolic compounds were identified and quantified by reversed phase liquid chromatography on both fruit extract and juice, over three years. The average polymerization degree of flavanols was estimated in fruit by phloroglucinolysis coupled to HPLC. Parental maps were built using SSR and SNP markers and used for the QTL analysis. Sixty-nine and 72 QTLs were detected on 14 and 11 linkage groups of the female and male maps, respectively. A majority of the QTLs identified in this study are specific to this population, while others are consistent with previous studies. This study presents for the first time in apple, QTLs for the mean polymerization degree of procyanidins, for which the mechanisms involved remains unknown to this day. Identification of candidate genes underlying major QTLs was then performed in silico and permitted the identification of 18 enzymes of the polyphenol pathway and six transcription factors involved in the apple anthocyanin regulation. New markers were designed from sequences of the most interesting candidate genes in order to confirm their co-localization with underlying QTLs by genetic mapping. Finally, the potential use of these QTLs in breeding programs is discussed.
Research center :
INRA Angers-Nantes, Université d'Angers
Disciplines :
Genetics & genetic processes
Author, co-author :
Verdu, Cindy ;  Laboratoire SONAS, Université d'Angers - IRHS, INRA d'Angers
Guyot, Sylvain;  INRA Rennes
Childebrand, Nicolas;  IRHS, INRA Angers
Bahut, Muriel;  Université d'Angers
Celton, Jean-Marc;  IRHS, INRA Angers
Gaillard, Sylvain;  IRHS, INRA Angers
Lasserre-Zuber, Pauline;  IRHS, INRA Angers
Troggio, Michela;  Fondazione Edmund Mach, S. Michele all’Adige (TN), Italia
Guilet, David;  Université Angers
Laurens, François;  IRHS, INRA Angers
Language :
English
Title :
QTL analysis and candidate gene mapping for the polyphenol content in cider apple
Publication date :
01 October 2014
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Volume :
9
Issue :
10
Pages :
e107103
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Cartographie génétique des composés phénoliques de la pomme
Funders :
projet Innovacidre; SFR QUASAV
Available on ORBi :
since 02 November 2014

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