No full text
Unpublished conference/Abstract (Scientific congresses and symposiums)
Effect of an anti-termite treatment on hindgut content metaproteome
Bauwens, Julien; Fossépré, Marie; De Pauw, Edwin et al.
2014Seventh International Symposium on Molecular Insect Science
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Termite; Boron; Tannins; Hindgut; Proteomics
Abstract :
[en] Introduction Termites are studied for many aspects of their biology. Historically known as pests in regard of human activities, these insects were more recently intensively investigated in the biofuel context. Symbiotic interactions occurring along the termite gut are of particular interest for both of these scopes. In this study we compared hindgut metaproteome of termites fed with an anti-termite treatment and an untreated diet. Material & Methods Termites were fed with Whatman paper for 84 hours. For untreated and treated diet respectively, paper was moisten with water and a sub lethal suspension of tannins associated with boric acid (BAT). Termite hindgut content proteome was extracted and digested. Peptides were analyzed through nano-LC-ESI-MS/MS using an Orbitrap analyzer. Proteins identification was realized by Mascot search in a homemade termite protein sequences database. Non redundant peptides with a score above identity threshold were blasted against NCBI nr database and results of this blast were analyzed using MeGAn 5. Results High resolution mass spectrometry allowed identification of around 1500 and 1000 non redundant peptides respectively for water and ABT treatment. Peptides were parsed following their taxonomic and functional attribution in order to highlight differences in hindgut metabolism such as cellulose digestion or detoxification process. Identification of ubiquitous proteins also revealed differences in symbiotic populations balance. Conclusions Despite the sub lethal concentration for the anti-termite treatment, significant perturbation of hindgut metabolism was observed. Tannins are slightly repulsive for termites compared to boric acid alone, and this has to be taken in account. These results will be completed with biochemical assays, microscopic observations and 2D-DIGE MALDI-TOF/TOF experiment, analyzing potentially synergistic effect between boric acid and tannins.
Disciplines :
Entomology & pest control
Author, co-author :
Bauwens, Julien ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Fossépré, Marie
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Thevenon, Marie-France;  CIRAD
Francis, Frédéric  ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Language :
English
Title :
Effect of an anti-termite treatment on hindgut content metaproteome
Publication date :
16 July 2014
Event name :
Seventh International Symposium on Molecular Insect Science
Event date :
du 13 juillet 2014 au 16 juillet 2014
Audience :
International
Available on ORBi :
since 20 October 2014

Statistics


Number of views
137 (8 by ULiège)
Number of downloads
0 (0 by ULiège)

Bibliography


Similar publications



Contact ORBi