Dose-dependent RNAi-mediated geminivirus resistance in the tropical root crop cassava.

DNA, Viral/isolation & purification; Geminiviridae/pathogenicity; Gene Expression Regulation, Plant; Immunity, Innate; Manihot/genetics/immunology/metabolism/virology; Plant Diseases/genetics/virology; Plants, Genetically Modified/genetics/immunology/metabolism/virology; Plasmids; RNA Interference; RNA, Double-Stranded/metabolism; RNA, Small Interfering/metabolism; Viral Proteins/genetics/metabolism

Abstract :

[en] Cassava mosaic disease is a major constraint for cassava production in Africa, resulting in significant economic losses. We have engineered transgenic cassava with resistance to African cassava mosaic virus (ACMV), by expressing ACMV AC1-homologous hairpin double-strand RNAs. Transgenic cassava lines with high levels of AC1-homologous small RNAs have ACMV immunity with increasing viral load and different inoculation methods. We report a correlation between the expression of the AC1-homologous small RNAs and the ACMV resistance of the transgenic cassava lines. Characterization of the small RNAs revealed that only some of the hairpin-derived small RNAs fall into currently known small interfering RNA classes in plants. The method is scalable to stacking by targeting multiple virus isolates with additional hairpins.

Disciplines :

Biotechnology

Author, co-author :

Vanderschuren, Hervé ; Université de Liège - ULiège > Sciences agronomiques > Biologie végétale

Alder, Adrian

Zhang, Peng

Gruissem, Wilhelm

Language :

English

Title :

Dose-dependent RNAi-mediated geminivirus resistance in the tropical root crop cassava.

Publication date :

2009

Journal title :

Plant Molecular Biology

ISSN :

0167-4412

eISSN :

1573-5028

Publisher :

Kluwer Academic Publishers, Netherlands

Volume :

Issue :

Pages :

265-72

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 06 October 2014

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127

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101

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112

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153

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