Empowering biotechnology in southern Africa: establishment of a robust transformation platform for the production of transgenic industry-preferred cassava.
[en] Knowledge and technology transfer to African laboratories and farmers is an important objective for achieving food security and sustainable crop production on the sub-Saharan African continent. Cassava (Manihot esculenta Crantz) is a vital source of calories for more than a billion people in developing countries, and its potential industrial use for starch and bioethanol in the tropics is increasingly being recognized. However, cassava production remains constrained by the susceptibility of the crop to several biotic and abiotic stresses. For more than a decade, biotechnology has been considered an attractive tool to improve cassava as it substantially circumvents the limitations of traditional breeding, which is particularly time-consuming and tedious because of the high heterozygosity of the crop. A major constraint to the development of biotechnological approaches for cassava improvement has been the lack of an efficient and robust transformation and regeneration system. Despite some success achieved in genetic modification of the model cassava cultivar Tropical Manihot Series (TMS), TMS 60444, in some European and U.S. laboratories, the lack of a reproducible and robust protocol has not allowed the establishment of a routine transformation system in sub-Saharan Africa. In this study, we optimized a robust and efficient protocol developed at ETH Zurich to successfully establish transformation of a commercially cultivated South African landrace, T200, and compared this with the benchmark model cultivar TMS 60444. Results from our study demonstrated high transformation rates for both T200 (23 transgenic lines from 100 friable embryogenic callus (FEC) clusters) compared with TMS 60444 (32 transgenic lines from 100 FEC clusters). The success in transforming landraces or farmer-preferred cultivars has been limited, and the high transformation rate of an industry-preferred landrace in this study is encouraging for a feasible transformation program for cassava improvement in South Africa (SA), which can potentially be extended to other countries in southern Africa. The successful establishment of a robust cassava transformation and regeneration system in SA demonstrates the relevance of technology transfer to sub-Saharan Africa and highlights the importance of developing suitable and reliable techniques before their transfer to laboratories offering less optimal conditions.
Empowering biotechnology in southern Africa: establishment of a robust transformation platform for the production of transgenic industry-preferred cassava.
Publication date :
2013
Journal title :
New Biotechnology
ISSN :
1871-6784
eISSN :
1876-4347
Publisher :
Elsevier, Netherlands
Volume :
30
Issue :
2
Pages :
136-43
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2012 Elsevier B.V. All rights reserved.
Cassava for Food and Energy Security 2008, FAO Media Center, (accessed June 2011). http://www.fao.org/newsroom/en/nes/2008/1000899/index.htlm.
Burns A., et al. The drought, war and famine crop in a changing world. Sustainability 2010, 2:3572-3607.
Hillocks R.J. Cassava in Africa. Cassava: Biology, Production and Utilization 2002, 41-54. CABI Publishing. R.J. Hillocks, J.M. Thresh, A.C. Bellotti (Eds.).
Balat M., Balat H. Recent trends in global production and utilization of bio-ethanol fuel. Appl. Energy 2009, 86:2273-2282.
Jansson C., et al. Cassava, a potential biofuel crop in (the) People's Republic of China. Appl. Energy 2009, 86:S95-S99.
Daphne P. Cassava: a South African venture. Optima 1980, 1:61-68.
Mathews C. Cassava production by small holder farmers in Mpumalanga: a case study. South African Society for Crop Production Congress 2000.
Berry S.D., Rey M.E.C. Molecular evidence for diverse populations of cassava-infecting begomoviruses in southern Africa. Arch. Virol. 2001, 146:1795-1802.
Patil B.L., Fauquet C.M. Cassava mosaic geminiviruses: actual knowledge and perspectives. Mol. Plant Pathol. 2009, 10:685-701.
Legg J.P., et al. Cassava mosaic virus disease in East and Central Africa: epidemiology and management of a regional pandemic. Adv. Virus Res. 2006, 67:355-418.
Mignouna H.D., Dixon A.G.O. Genetic relationships among cassava clones with varying levels of resistance to African mosaic disease using RAPD markers. Afr. J. Root Tuber Crops 1997, 2:28-32.
Okogbenin E., et al. Marker-assisted introgression of resistance to cassava mosaic disease into Latin American germplasm for the genetic improvement of cassava in Africa. Crop Sci. 2007, 47:1895-1904.
Raji A.A., et al. Screening landraces for additional sources of field resistance to cassava mosaic disease and green mite for integration into the cassava improvement program. J. Integr. Plant Biol. 2008, 50:311-318.
Ceballos H., et al. Cassava breeding: opportunities and challenges. Plant Mol. Biol. 2004, 56:503-516.
Brink J.A., et al. Plant biotechnology: a tool for development in Africa. Electron. J. Biotechnol. 1998, 1:1-12.
Thomson J.A. The role of biotechnology for agricultural sustainability in Africa. Philos. Trans. R. Soc. Lond. 2007, 363:905-913.
Vanderschuren H., et al. Engineering resistance to geminiviruses - review and perspectives. Plant Biotechnol. J. 2007, 5:207-220.
Vanderschuren H., et al. Transgenic cassava resistance to African cassava mosaic virus is enhanced by viral DNA-A bidirectional promoter-derived siRNAs. Plant Mol. Biol. 2007, 64:549-557.
Chellappan P., et al. Broad spectrum resistance to ssDNA viruses associated with transgene-induced gene silencing in cassava. Plant Mol. Biol. 2004, 56:601-6011.
Zhang P., et al. Resistance to cassava mosaic disease in transgenic cassava expressing antisense RNAs targeting virus replication genes. Plant Biotechnol. J. 2005, 3:385-397.
Vanderschuren H., et al. Dose-dependent RNAi-mediated geminivirus resistance in the tropical root crop cassava. Plant Mol. Biol. 2009, 70:265-272.
Donald Danforth Plant Science Center. News Release (Danforth Center Cassava Update, Friday May 26th, 2006): (last accessed on 10/11/2011). http://www.danforth%20center.org/wordpress/%3Fpage_id=395%26pid=1474.
Li H.Q., et al. Genetic transformation of cassava (Manihot esculenta Crantz). Nat. Biotechnol. 1996, 14:736-740.
Schopke C., et al. Regeneration of transgenic cassava plants (Manihot esculenta Crantz) from microbombarded embryogenic suspension cultures. Nat. Biotechnol. 1996, 14:731-735.
Hankoua B.B., et al. Production of the first transgenic cassava in Africa via direct shoot organogenesis from friable embryogenic calli and germination of maturing somatic embryos. Afr. J. Biotechnol. 2006, 5:1700-1712.
Bull S.E., et al. Cassava: constraints to production and the transfer of biotechnology to African laboratories. Plant Cell Rep. 2011, 30:779-787.
Liu J., et al. Cassava genetic transformation and its application in breeding. J. Integr. Plant Biol. 2011, 53:552-569.
Schreuder M.M., et al. Efficient production of transgenic plants by Agrobacterium-mediated transformation of cassava. Euphytica 2001, 120:35-42.
Bull S.E., et al. Agrobacterium mediated transformation of friable embryogenic calli and regeneration of transgenic cassava. Nat. Protoc. 2009, 4:1845-1854.
Zhang P., Gruissem W. Production of transgenic cassava (Manihot esculenta Crantz). Transgenic Crops of the World - Essential Protocols 2004, Kluwer Academic Publishers, pp. 301-319.
Rossin C.B., Rey M.E.C. Effect of explant source and auxins on somatic embryogenesis of selected cassava (Manihot esculenta Crantz) cultivars. S. Afr. J. Bot. 2010, 77:59-67.
Greshoff P., Doy C.H. Derivation of a haploid cell line from Vitis vinifera and importance of stage of meiotic development of anthers for haploid culture of this and other genera. Z. Pflanzenphysiol. 1974, 73:132-141.
Murashige T., Skoog F. A revised medium for rapid growth and bio assays with tobacco tissues cultures. Physiol. Plant. 1962, 15:473-1962.
Doyle J.J., Doyle J.L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 1987, 19:11-15.
Briddon R.W., et al. Recommendations for the classification and nomenclature of the DNA-b satellites of begomoviruses. Arch Virol 2008, 153:763-781.
Calvert L.A., Thresh J.M. The viruses and virus diseases of cassava. Cassava: Biology, Production and Utilization 2002, 236-260. CABI Publishing. R.J. Hillocks, J.M. Thresh, A.C. Bellotti (Eds.).
Baba A., et al. Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta). Plant Sci. 2008, 175:717-723.
Li H.Q., et al. Regeneration of cassava plants via shoot organogenesis. Plant Cell Rep. 1998, 17:410-414.
Taylor N.J., et al. Improved procedures for producing embryogenic tissues of African cassava genotypes: implications for genetic transformation. Afr. J. Root Tuber Crops 1997, 2:200-204.
Raemakers C.J.J.M., et al. Progress made in FEC transformation of cassava. Euphytica 2001, 120:15-24.
Koehorst-Van Putten H.J., et al. Field testing and exploitation of genetically modified cassava with low-amylose or amylose-free starch in Indonesia. Transgenic Res. 2012, 21:39-50.
Zhang P., et al. Improvement of cassava shoot organogenesis by the use of silver nitrate in vitro. Plant Cell Tissue Organ 2001, 67:47-54.
Bothma G., et al. GMOs in Africa: opportunities and challenges in South Africa. GM Crops 2010, 1:175-180.
Shepherd D.N., et al. Maize streak virus-resistant transgenic maize: a first for Africa. Plant Biotechnol. 2007, 5:759-767.
Aerni P. Mobilizing science and technology for development: the case of the Cassava Biotechnology Network (CBN). AgBioForum 2006, 9:1-14.
Niklaus M., et al. Robust transformation and regeneration of transgenic cassava using the neomycin phosphotransferase gene as aminoglycoside resistance marker gene. GM Crops 2011, 2:193-200.