Article (Scientific journals)
Identification of undeclared sources of animal origin in canine dry foods used in dietary elimination trials
Ricci, Rebecca; Granato, A; Vascellari, M et al.
2013In Journal of Animal Physiology and Animal Nutrition, 97, p. 32-38
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Keywords :
adverse food reaction; allergens; contamination; pet food; microscopy analysis; PCR
Abstract :
[en] Failure to respond to commercial limited antigen diets can occur in dogs kept on a dietary trial for the diagnosis of adverse food reaction (AFR). The aim of this study was to assess twelve canine dry limited antigen diets (eleven novel protein diets and one hydrolysed diet) for potential contamination by ingredients of animal origin not mentioned on the label. The validity of the two methods adopted for the detection of such food antigens was also evaluated. Each dietary product was analysed by microscopy analysis using the official method described in Commission Regulation EC 152/2009 with the aim of identifying bone fragments of different zoological classes (mammalian, avian and fish) and by polymerase chain reaction (PCR) for the identification of DNA of animal origin. Discrepancies between the results obtained by PCR and/or microscopy analysis and the ingredients listed on pet food packages were found. Only in two pet foods did the results of both analyses match the ingredients listed on the label. In the remaining ten samples, microscopy detected bone fragments from one or two unpredicted zoological classes, revealing avian fragments in six of ten samples followed by those of fish in five of ten and mammalian fragments in four of ten. In two samples, microscopy analysis identified a contamination that would have otherwise passed unobserved if only PCR had been used. However, PCR confirmed the presence of all the zoological classes detected by microscopy and also identified the DNA of an additional unexpected zoological class in two samples. Dogs might fail to respond to commercial limited antigen diets because such diets are contaminated with potential allergens. Both PCR and microscopy analysis are required to guarantee the absence of undeclared animal sources in pet foods. Before ruling out AFR, a novel protein home-made diet should be considered if the dog is unresponsive to a commercial regimen.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ricci, Rebecca;  Dpt of Animal Medicine, Production and Health, University of Padua, Italy
Granato, A;  Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy
Vascellari, M;  stituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy
Boscarato, M;  Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy
Palagiano, Cl.;  Dpt of Animal Medicine, Production and Health, University of Padua, Italy
Andrighetto, I;  Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy
Diez, Marianne ;  Université de Liège - ULiège > Département de productions animales > Nutrition des animaux de compagnie
Mutinelli, F;  Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy
Language :
English
Title :
Identification of undeclared sources of animal origin in canine dry foods used in dietary elimination trials
Publication date :
2013
Journal title :
Journal of Animal Physiology and Animal Nutrition
ISSN :
0931-2439
eISSN :
1439-0396
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
97
Pages :
32-38
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Dpt of Animal Medicine, Production and Health, University of Padua, Legnaro (PD), Italy
Available on ORBi :
since 23 September 2014

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