Reference : Polymer topology revealed by ion mobility coupled with mass spectrometry
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/171848
Polymer topology revealed by ion mobility coupled with mass spectrometry
English
Morsa, Denis mailto [Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
Defize, Thomas mailto [Université de Liège - ULiège > > Centre d'études et de rech. sur les macromolécules (CERM) >]
Dehareng, Dominique mailto [Université de Liège - ULiège > > Centre d'ingénierie des protéines >]
Jérôme, Christine mailto [Université de Liège - ULiège > Département de chimie (sciences) > Chimie des macromolécules et des matériaux organiques (CERM) >]
De Pauw, Edwin mailto [Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
Sep-2014
Analytical Chemistry
American Chemical Society
86
19
9693–9700
Yes (verified by ORBi)
International
0003-2700
1520-6882
Washington
DC
[en] Hyperbranched and star shaped polymers have raised tremendous interests
because of their unusual structural and photochemical properties which provide them potent
applications in various domains, namely in the biomedical field. In this context, the
development of adequate tools aiming to probe particular three-dimensional features of such
polymers is of crucial importance. In this present work, ion mobility coupled with mass
spectrometry was used to experimentally derive structural information related to cationized
linear and star-shaped poly-ε-caprolactones as a function of their charge state and chain
length. Two major conformations were observed and identified using theoretical modeling:
(1) near spherical conformations whose size is invariant with the polymer topology for long
and lightly charged chains and (2) elongated conformations whose size varies with the
polymer topology for short and highly charged chains. These conformations were further
confirmed by collisional activation experiments based on the ejection thresholds of the coordinated cations that vary according to the elongation amplitude of the polymer chains.
Finally, a comparison between solution and gas-phase conformations highlights a compaction
of the structure with a loss of specific chain arrangements during the ionization and
desolvation steps of the electrospray process, fueling the long-time debated question related to
the preservation of the analyte structure during the transfer into the mass spectrometer.
Giga-Systems Biology and Chemical Biology ; Centre d'Ingénierie des Protéines - CIP ; CERM
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/171848
10.1021/ac502246g
http://pubs.acs.org/doi/abs/10.1021/ac502246g

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