[en] Herein we report on the synthesis and sensor activity of a novel pH sensitive probe designed as highly water-soluble fluorescent micelles by grafting of 1,8-naphthalimide–rhodamine bichromophoric FRET system (RNI) to the PMMA block of a well-defined amphiphilic diblock copolymer—poly(methyl methacrylate)–b-poly(methacrylic acid) (PMMA48–b-PMAA27). The RNI-PMMA48–b-PMAA27 adduct is capable of self-assembling into micelles with a hydrophobic PMMA core, containing the anchored fluorescent probe, and a hydrophilic shell composed of PMAA block. Novel fluorescent micelles are able to serve as a highly sensitive pH probe in water and to internalize successfully HeLa and HEK cells. Furthermore, they showed cell specificity and significantly higher photostability than that of a pure organic dye label such as BODIPY. The valuable properties of the newly prepared fluorescent micelles indicate the high potential of the probe for future biological and biomedical applications.
Research Center/Unit :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Materials science & engineering Chemistry
Author, co-author :
Georgiev, N. I.; University of Chemical Technology and Metallurgy, Sofia, Bulgaria, Department of Organic Synthesis
Bryaskova, Rayne; University of Chemical Technology and Metallurgy, Sofia, Bulgaria, Department of Polymer Engineering
Tzoneva, R.; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Science, Sofia, Bulgaria
Ugrinova; Institute of Molecular Biology, Bulgarian Academy of Science, Sofia, Bulgaria
Detrembleur, Christophe ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Miloshev, S.; University of Chemical Technology and Metallurgy, Sofia, Bulgaria, Department of Polymer Engineering
Asiri, A. M.; King Abdulaziz University, Saudi Arabia, Chemistry Department, Faculty of Sciences > King Abdulaziz University,Saudi Arabia, Center of Excellence for Advanced Materials Research (CEAMR)
Qusti, A. H.; King Abdulaziz University, Saudi Arabia, Chemistry Department, Faculty of Sciences
Bojinov, V. B.; University of Chemical Technology and Metallurgy, Sofia, Department of Organic Synthesis > King Abdulaziz University, Saudi Arabia, Chemistry Department, Faculty of Sciences
Language :
English
Title :
A novel pH sensitive water soluble fluorescent nanomicellar sensor for potential biomedical applications
Publication date :
01 November 2013
Journal title :
Bioorganic and Medicinal Chemistry
ISSN :
0968-0896
eISSN :
1464-3391
Publisher :
Elsevier Science, Oxford, United Kingdom
Volume :
21
Issue :
21
Pages :
6292-6303
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique BNSF - Bulgarian National Science Fund The Research Program of the University of Chemical Technology and Metallurgy, Sofia, Bulgaria
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