[en] Aim: Produce biodegradable nanoparticles to target antigen-presenting cells and evaluate their potential to be used as a vaccine delivery system. Materials & methods: Untargeted PEGylated PLGA-based nanoparticles and mannose-grafted nanoparticles were formulated and physicochemically characterized. Immortalized and primary antigen-presenting cells were used to study nanoparticle internalization patterns. The endocytic pathways and intracellular trafficking followed by nanoparticles were also investigated. Results & discussion: Nanoparticles displayed mannose residues available for binding at the nanoparticle surface. Different nanoparticle internalization patterns by immortalized and primary antigen presenting cells were verified. Macropinocytosis, clathrin-mediated endocytosis, caveolin- and lipid raft-dependent endocytosis are involved in nanoparticles internalization. Nanoparticles demonstrate both endo-lysosomal and cytosolic localizations and a tendency to accumulate nearby the endoplasmic reticulum. Conclusion & future perspective: The developed nanoparticles might drive antigens to be presented through MHC class I and II molecules to both CD8+ and CD4+ T cells, favoring a complete and coordinated immune response.
Research Center/Unit :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Chemistry Materials science & engineering
Author, co-author :
Silva, Joana M.; Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia > Université catholique de Louvain (UCL), Brussels, Louvain Drug Research Institute (LDRI), Advanced Drug Delivery & Biomaterials
Vandermeulen, Gaëlle; Université catholique de Louvain (UCL), Brussels > Louvain Drug Research Institute (LDRI) > Advanced Drug Delivery & Biomaterials
Oliveira, Vanessa G.; Faculdade de Medicina da Universidade de Lisboa, Instituto de Medicina Molecular
Pinto, Sandra N.; Universidade de Lisboa, Centro de Química-Física Molecular & Institute of Nanoscience and Nanotechnology, Instituto Superior Técnico
Rodrigues, Catarina; Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia
Salgado, Ana; Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia
Afonso, Carlos A.; Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia
Viana, Ana S.; Faculdade de Ciências da Universidade de Lisboa, Centro de Química e Bioquímica
Jérôme, Christine ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie des macromolécules et des matériaux organiques (CERM)
Silva, Liana C.; Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia
Graca, Luiis; Faculdade de Medicina da Universidade de Lisboa, Instituto de Medicina Molecular
Préat, Véronique; Université catholique de Louvain (UCL), Brussels > Louvain Drug Research Institute (LDRI) > Advanced Drug Delivery & Biomaterials
Florindo, Helena F.; Universidade de Lisboa, Instituto de Investigação do Medicamento, Faculdade de Farmácia
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