Identification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota
[en] A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-β-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions.
Disciplines :
Microbiology
Author, co-author :
Martin, Marjolaine ; Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Biver, Sophie ; Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Steels, Sébastien ; Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Barbeyron, Tristan
Jam, Murielle
Portetelle, Daniel ; Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Michel, Gurvan
Vandenbol, Micheline ; Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Language :
English
Title :
Identification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota
Publication date :
August 2014
Journal title :
Applied and Environmental Microbiology
ISSN :
0099-2240
eISSN :
1098-5336
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
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