Article (Scientific journals)
Cell-line specific radiosensitizing effect of zalcitabine (DDC)
Coucke, Philippe; Li, Ye-Xiong; Copaceanu, Marie-Laure et al.
1997In Acta Oncologica
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Keywords :
Cell-line; Zalcitabine; Radiosensitizing
Abstract :
[en] The potential of zalcitabine (ddC) to act as an ionizing radiation response modifier was tested on exponentially growing human cancer cells in vitro. Two human cell lines, WiDr (colon) and MCF-7 (breast) were exposed to ddC at 10 p M concentration for various lengths of tide (18, 24, 48 and 72 h). On the WiDr cell line the dual effect of concentration and duration of exposure prior to irradiation was investigated. Experimental endpoints were clonogenicity and viability, as measured by colony formation assay (CFA) and MTT assay respectively. The impact on cell-cycle distribution prior to irradiation was assessed by flow cytometry using a double labeling technique (propidium iodide and bromodeoxyuridine pulse label). A significant reduction in surviving fraction and viability was observed for WiDr-cells irradiated after pre-exposure to 10 pM for 18, 48 and 72 h as compared to corresponding irradiated controls. At lower concentrations (1 and 5 pM), the radiosensitizing effect was only significant after a 72-h exposure (assessed by CFA). For MCF-7, ddC induced a significant modification of the dose response only with 24 and 48 h preincubation. However, the overall effect was less pronounced as compared to WiDr. Cell-cycle analysis showed accumulation in S-phase, 48 and 72 h after treatment with 10 pM ddC in the WiDr cells, with a progressive shift to late S-phase as shown by the biparametric analysis. The degree of radiosensitization is cell-line dependent with the most important sensitization observed on the most <<radioresistant cell line>>, ix., the cell line with the lowest alpha value and highest SF 2 (WiDr). For WiDr, radiosensitization by ddC depends on the duration of exposure and the concentration of the drug. Received 29 February 1996 Accepted 10 December 1996
Disciplines :
Hematology
Oncology
Author, co-author :
Coucke, Philippe  ;  Centre Hospitalier Universitaire Vaudois > Laboratory of Radiobiology, Department of Radiation-Oncology > Lausanne, Switzerland
Li, Ye-Xiong;  Centre Hospitalier Universitaire Vaudois > Laboratory of Radiobiology, Department of Radiation-Oncology > Lausanne, Switzerland
Copaceanu, Marie-Laure;  Centre Hospitalier Universitaire Vaudois > Laboratory of Radiobiology, Department of Radiation-Oncology > Lausanne, Switzerland
Paschoud, Nicolas;  Centre Hospitalier Universitaire Vaudois > Laboratory of Radiobiology, Department of Radiation-Oncology > Lausanne, Switzerland
Cottin, Eliane;  Centre Hospitalier Universitaire Vaudois > Laboratory of Radiobiology, Department of Radiation-Oncology > Lausanne, Switzerland
Ozsahin, Mahmut;  Centre Hospitalier Universitaire Vaudois > Laboratory of Radiobiology, Department of Radiation-Oncology > Lausanne, Switzerland
Mirimanoff;  Centre Hospitalier Universitaire Vaudois > Laboratory of Radiobiology, Department of Radiation-Oncology > Lausanne, Switzerland
Language :
English
Title :
Cell-line specific radiosensitizing effect of zalcitabine (DDC)
Publication date :
1997
Journal title :
Acta Oncologica
ISSN :
0284-186X
eISSN :
1651-226X
Publisher :
Taylor & Francis, Oslo, Norway
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 15 July 2009

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