[en] The hermaphroditic gastropod Lymnaea stagnalis is proposed as a candidate species for the development of OECD guidelines for testing of the reprotoxicity of chemicals, including endocrine active substances (EASs). Up to now, only a few putative EASs have been tested for their reproductive toxicity in this species. In this study, we investigate the effects of four EASs with different affinities to the vertebrate estrogen and androgen receptors (chlordecone as an estrogen; cyproterone acetate, fenitrothion and vinclozolin as anti-androgens) on the reproduction of L. stagnalis in a 21-day semi-static test. Testosterone and 17α-ethinylestradiol (EE2) were used as the reference compounds. The tested EASs had no significant effect on growth and survival at the tested concentration ranges (ng to μg/L). Classical reproduction endpoints (i.e., oviposition and fecundity) were not responsive to the tested chemicals, except for chlordecone and 17α-ethinylestradiol, which hampered reproduction from 19.6 μg/L and 17.6 μg/L, respectively. The frequency of polyembryonic eggs, used as an additional endpoint, demonstrated the effects of all compounds except EE2. The molecular pathways, which are involved in such reproduction impairments, remain unknown. Our results suggest that egg quality is a more sensitive endpoint as compared to other reproductive endpoints commonly assessed in mollusk toxicity tests.
Disciplines :
Environmental sciences & ecology
Author, co-author :
Giusti, Arnaud ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Département de Biologie, Ecologie et Evolution
Lagadic, Laurent; Institut Scientifique de Recherche Agronomique - INRA > UMR0985 Ecologie et Santé des Ecosystèmes > Equipe Ecotoxicologie et Qualité des Milieux Aquatiques
Barsi, Alpar; Institut Scientifique de Recherche Agronomique - INRA > UMR0985 Ecologie et Santé des Ecosystèmes > Equipe Ecotoxicologie et Qualité des Milieux Aquatiques
Thomé, Jean-Pierre ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie
Joaquim-Justo, Célia ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie
Ducrot, Virginie; Institut Scientifique de Recherche Agronomique - INRA > UMR0985 Ecologie et Santé des Ecosystèmes > Equipe Ecotoxicologie et Qualité des Milieux Aquatiques
Language :
English
Title :
Investigating apical adverse effects of four endocrine active substances in the freshwater gastropod Lymnaea stagnalis
FP7 - 238148 - CREAM - Mechanistic Effect Models for the Ecological Risk Assessment of Chemicals
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture FRFC - Fonds de la Recherche Fondamentale Collective French funds from the Agency for Food, Environmental and Occupational Health Safety under the “Programme Environnement-Santé-Travail de l'ANSES”, contract CREOLE, number EST/2011/1/190 CE - Commission Européenne
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