In vitro embryo production in goats: slaughterhouse and laparoscopic ovum pick up 2 (LOPU) derived oocytes have different kinetics and requirements regarding maturation 3 media
[en] A total of 3427 goat oocytes were used in this study in order to identify possible 24 differences during in vitro embryo production from slaughterhouse or laparoscopic ovum 25 pick up (LOPU) oocytes. In Experiment 1, one complex, one semi defined and one simplified 26 in vitro maturation (IVM) media were compared using slaughterhouse oocytes. In 27 Experiment 2, we checked the effect of oocyte origin (slaughterhouse or LOPU) on the 28 kinetics of maturation (18 vs. 22 vs. 26 h) when submitted to semi defined or simplified 29 media. In Experiment 3, we determined the differences in embryo development between 30 slaughterhouse and LOPU oocytes when submitted to both media and then to in vitro 31 fertilization (IVF) or parthenogenetic activation (PA). Embryos from all groups were vitrified 32 and their viability evaluated in vitro after thawing. In Experiment 1, no difference (P>0.05) 33 was detected among treatments for maturation rate (MII; 88% on average), cleavage (72%), 34 blastocyst from the initial number of cumulus oocyte complexes (COC; 46%) or from the 35 cleaved ones (63%), hatching rate (69%) and the total number of blastomeres (187). In 36 Experiment 2, there was no difference of MII rate between slaughterhouse oocytes cultured 37 for 18 or 22 h, whereas the MII rate increased significantly (P<0.05) between 18 and 22 h for 38 LOPU oocytes in the simplified medium. Moreover, slaughterhouse oocytes cultured in 39 simplified medium matured significantly faster than LOPU oocytes at 18 and 22 h (P<0.05). 40 In Experiment 3, cleavage rate was significantly greater (P<0.001) in all four groups of 41 embryos produced by PA than IVF. Interestingly, PA reached similar rates for slaughterhouse 42 oocytes cultured in both media, but improved (P<0.05) cleavage rate of LOPU oocytes. 43 Slaughterhouse oocytes had acceptable cleavage rate after IVF (~67%), whereas LOPU 44 oocytes displayed a lower one (~38%), in contrast to cleavage after PA. The percentage of 45 blastocysts in relation to cleaved embryos was not affected by the origin of the oocytes 46 (P>0.05). Therefore, slaughterhouse oocytes developed a greater proportion of blastocysts than LOPU ones, expressed as the percentage of total COC entering to IVM. Vitrified-thawed 48 blastocysts presented similar survival and hatching rates between the oocyte origin, media or 49 method of activation. In conclusion, slaughterhouse and LOPU derived oocytes may have 50 different IVM kinetics and require different IVM-IVF conditions. Although the IVM and IVF 51 systems still need improvements in order to enhance embryo yield, the in vitro development 52 (IVD) step is able to generate good quality embryos from LOPU derived oocytes.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Souza-Fabjan, JM; Institut Scientifique de Recherche Agronomique - INRA > UMR7247, Physiologie de la Reproduction et des Comportements, INRA, CNRS, 10 Université de Tours, Haras Nationaux. 37380 Nouzilly, France
Locatelli, Y; Institut Scientifique de Recherche Agronomique - INRA > UMR7247, Physiologie de la Reproduction et des Comportements, INRA, CNRS, 10 Université de Tours, Haras Nationaux. 37380 Nouzilly, France
Duffard, N; Institut Scientifique de Recherche Agronomique - INRA > UMR7247, Physiologie de la Reproduction et des Comportements, INRA, CNRS, 10 Université de Tours, Haras Nationaux. 37380 Nouzilly, France
Corbin, E; Institut Scientifique de Recherche Agronomique - INRA > UMR7247, Physiologie de la Reproduction et des Comportements, INRA, CNRS, 10 Université de Tours, Haras Nationaux. 37380 Nouzilly, France
Touzé, JL
Perreau, Ch
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Freitas, VJ
Mermillod, P; Institut Scientifique de Recherche Agronomique - INRA > UMR7247, Physiologie de la Reproduction et des Comportements, INRA, CNRS, 10 Université de Tours, Haras Nationaux. 37380 Nouzilly, France
Language :
English
Title :
In vitro embryo production in goats: slaughterhouse and laparoscopic ovum pick up 2 (LOPU) derived oocytes have different kinetics and requirements regarding maturation 3 media
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