[en] Uncoupling proteins (UCPs) are mitochondrial inner membrane proteins sustaining an inducible proton conductance. They weaken the proton electrochemical gradient built up by the mitochondrial respiratory chain. Brown fat UCP1 sustains a free fatty acid (FA)-induced purine nucleotide (PN)-inhibited proton conductance. Inhibition of the proton conductance by PN has been considered as a diagnostic of UCP activity. However, conflicting results have been obtained in isolated mitochondria for UCP homologues (i.e., UCP2, UCP3, plant UCP, and protist UCP) where the FFA-activated proton conductance is poorly sensitive to PN under resting respiration conditions. Our recent work clearly indicates that the membranous coenzyme Q, through its redox state, represents a regulator of the inhibition by PN of FFA-activated UCP1 homologues under phosphorylating respiration conditions. Several physiological roles of UCPs have been suggested, including a control of the cellular energy balance as well as the preventive action against oxidative stress. In this paper, we discuss new information emerging from comparative proteomics about the impact of UCPs on mitochondrial physiology, when recombinant UCP1 is expressed in yeast and when UCP2 is over-expressed in hepatic mitochondria during steatosis
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Sluse, Francis ; Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
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Bibliography
Klingenberg, M., Mechanism and evolution of the uncoupling protein of brown adipose tissue (1990) Trends Biochem. Sci., 15, pp. 108-112
Ledesma, A., de Lacoba, M.G., Rial, E., The mitochondrial uncoupling proteins (2002) Genome Biol., 3, pp. 1-9
Jarmuszkiewicz, W., Sluse-Goffart, C.M., Vercesi, A.E., Sluse, F.E., Alternative oxidase and uncoupling protein: thermogenesis versus cell energy balance (2001) Biosci. Rep., 21, pp. 213-222
Skulachev, V.P., Role of uncoupled and non-coupled oxidations in maintenance of safety low levels of oxygen and its one-electron reductants (1996) Q. Rev. Biophys., 29, pp. 9895-9901
Sluse, F.E., Jarmuszkiewicz, W., Uncoupling proteins outside the animal and plant kingdoms: functional and evolutionary aspects (2002) FEBS Lett., 510, pp. 117-120
Jarmuszkiewicz, W., Almeida, A.M., Sluse-Goffart, C.M., Sluse, F.E., Vercesi, A.E., Linoleic acid-induced activity of plant uncoupling mitochondrial protein in purified tomato fruit mitochondria during resting, phosphorylating, and progressively uncoupled respiration (1998) J. Biol. Chem., 273, pp. 34882-34886
Jaburek, M., Varecha, M., Gimeno, R.E., Dembski, M., Jezek, P., Zhang, M., Burn, P., Garlid, K.D., Transport function and regulation of mitochondrial uncoupling proteins 2 and 3 (1999) J. Biol. Chem., 274, pp. 26003-26007
Borecky, J., Maia, I.G., Costa, A.D., Jezek, P., Chaimovich, H., de Andrade, P.B., Vercesi, A.E., Arruda, P., Functional reconstitution of Arabidopsis thaliana plant uncoupling mitochondrial protein (AtPUMP1) expressed in Escherichia coli (2001) FEBS Lett., 505, pp. 240-244
Zackova, M., Skobisova, E., Urbankova, E., Jezek, P., Activating omega-6 unsaturated fatty acids and inhibitory purine nucleotides are high affinity ligands for novel mitochondrial uncoupling proteins UCP2 and UCP3 (2003) J. Biol. Chem., 278, pp. 20761-20769
Considine, M.J., Goodman, M., Echtay, K.S., Laloi, M., Whelan, J., Brand, M.D., Sweetlove, L.J., Superoxide stimulates a proton leak in potato mitochondria that is related to the activity of uncoupling protein (2003) J. Biol. Chem., 278, pp. 22298-22302
Echtay, K.S., Esteves, T.C., Pakay, J.L., Jekabson, M.B., Lamber, A.J., Portero-Otin, M., Pamplona, R., Brand, M.D., A signalling role for 4-hydroxy-2-nonenal in regulation of mitochondrial uncoupling (2003) EMBO J., 22, pp. 4103-4110
Jekabsons, M.B., Echtay, K.S., Brand, M.D., Nucleotide binding to human uncoupling protein-2 refolded from inclusion bodies (2002) Biochem. J., 366, pp. 565-571
Klingenberg, M., Echtay, K.S., Uncoupling proteins: the issues from a biochemist point of view (2001) Biochim. Biophys. Acta, 1504, pp. 128-143
Jarmuszkiewicz, W., Almeida, A.M., Sluse-Goffart, C.M., Sluse, F.E., Vercesi, A.E., Linoleic acid-induced activity of plant uncoupling mitochondrial protein in purified tomato fruit mitochondria during resting, phosphorylating, and progressively uncoupled respiration (1998) J. Biol. Chem., 273, pp. 34882-34886
Rial, E., Gonzalez-Barroso, M., Fleury, C., Iturrizaga, S., Sanchis, D., Jimenez-Jimenez, J., Ricquier, D., Bouillaud, F., Retinoids activate proton transport by the uncoupling proteins UCP1 and UCP2 (1999) EMBO J., 18, pp. 5827-5833
Zhang, C.Y., Hagen, T., Mootha, V.K., Slieker, L.J., Lowell, B.B., Assessment of uncoupling activity of uncoupling protein 3 using a yeast heterologous expression system (1999) FEBS Lett., 449, pp. 129-134
Hourton-Cabassa, C., Mesneau, A., Miroux, B., Roussaux, J., Ricquier, D., Zachowski, A., Moreau, F., Alteration of plant mitochondrial proton conductance by free fatty acids (2002) J. Biol. Chem., 277, pp. 41533-41538
Nicholls, D.G., Rial, E., A history of the first uncoupling protein, UCP1 (1999) J. Bioenerg. Biomembr., 31, pp. 399-418
Douette, P., Navet, R., Bouillenne, F., Brans, A., Sluse-Goffart, C.M., Matagne, A., Sluse, F.E., Secondary-structure characterization by far-UV CD of highly purified uncoupling protein 1 expressed in yeast (2004) Biochem. J., 380, pp. 139-145
Douette, P., Gerkens, P., Navet, R., Leprince, P., De Pauw, E., Sluse, F.E., Uncoupling protein 1 affects the yeast mitoproteome and oxygen free radicals production (2006) Free Radic. Biol. Med., 40, pp. 303-315
Jezek, P., Possible physiological roles of mitochondrial uncoupling proteins-UCPn (2002) Int. J. Biochem. Cell Biol., 34, pp. 1190-1206
Chavin, K.D., Yang, S.-Q., Lin, H.Z., Chatham, J., Chacko, V.P., Hoeck, J.B., Walajtys-Rode, E., Diehl, A.M., Obesity induces expression of uncoupling protein 2 in hepatocytes and promotes liver ATP depletion (1999) J. Biol. Chem., 274, pp. 5692-5700
Stuart, J.A., Harper, J.A., Brindle, K.M., Jekabsons, M.B., Brand, M.D., Physiological levels of mammalian uncoupling protein 2 do not uncouple yeast mitochondria (2001) J. Biol. Chem., 276, pp. 18633-186339
Heidkaemper, D., Winkler, E., Muller, V., Frischmuth, K., Liu, Q., Caskey, T., Klingenberg, M., The bulk of UCP3 expressed in yeast cells is incompetent for a nucleotide regulated H+ transport (2000) FEBS Lett., 480, pp. 265-270
Stuart, J.A., Harper, J.A., Brindle, K.M., Jekabson, M.B., Brand, M.D., Artifactual uncoupling by uncoupling protein 3 in yeast mitochondria at the concentrations found in mouse and rat skeletal-muscle mitochondria (2001) Biochem. J., 356, pp. 779-789
Jarmuszkiewicz, W., Almeida, A.M., Vercesi, A.E., Sluse, F.E., Sluse-Goffart, C.M., Proton re-uptake partitioning between uncoupling protein and ATP synthase during benzohydroxamic acid-resistant state 3 respiration in tomato fruit mitochondria (2000) J. Biol. Chem., 275, pp. 13315-13320
Jarmuszkiewicz, W., Navet, R., Alberici, L.C., Douette, P., Sluse-Goffart, C.M., Sluse, F.E., Vercesi, A.E., Redox state of endogenous coenzyme Q modulates the inhibition of linoleic acid-induced uncoupling by guanosine triphosphate in isolated skeletal muscle mitochondrial (2004) J. Bioenerg. Biomembr., 36, pp. 493-502
Jarmuszkiewicz, W., Swida, A., Czarna, M., Antos, N., Sluse-Goffart, C.M., Sluse, F.E., In phosphorylating Acanthamoeba castellanii mitochondria the sensitivity of uncoupling protein activity to GTP depends on the redox state of quinone (2005) J. Bioenerg. Biomembr., 37, pp. 97-107
Navet, R., Douette, P., Puttine-Marique, F., Sluse-Goffart, C.M., Sluse, F.E., Activation and regulation of plant uncoupling protein in potato tuber mitochondria (2005) FEBS Lett., 579, pp. 4437-4442
Jarmuszkiewicz, W., Czarna, M., Sluse-Goffart, C.M., Sluse, F.E., The contribution of uncoupling protein and ATP synthase to state 3 respiration in Acanthamoeba castellanii mitochondria (2004) Acta Biochim. Pol., 51, pp. 533-538
Gonzalez-Barroso, M.M., Fleury, C., Arechaga, I., Zaragoza, P., Levi-Meyrueis, C., Raimbault, S., Ricquier, D., Rial, E., Activation of the uncoupling protein by fatty acids is modulated by mutations in the C-terminal region of the protein (1996) Eur. J. Biochem., 239, pp. 445-450
Echtay, K.S., Winkler, E., Klingenberg, M., Coenzyme Q is an obligatory factor for uncoupling protein function (2000) Nature, 408, pp. 609-613
Echtay, K.S., Winkler, E., Frischmuth, K., Klingenberg, M., Uncoupling proteins 2 and 3 are highly active H+ transporters and highly nucleotide sensitive when activated by coenzyme Q (ubiquinone) (2001) Proc. Natl. Acad. Sci. U. S. A., 98, pp. 1416-1421
Görg, A., Weiss, W., Dunn, M.J., Current two-dimensional electrophoresis technology for proteomics (2004) Proteomics, 4, pp. 3665-3685
Unlu, M., Morgan, M.E., Minden, J.S., Difference gel electrophoresis: a single method for detecting changes in protein extracts (1997) Electrophoresis, 18, pp. 2071-2077
Alban, A., David, S.O., Bjorkesten, L., Andersson, C., Sloge, E., Lewis, S., Currie, I.A., A novel experimental design for comparative two-dimensional gel analysis: two-dimensional difference gel electrophoresis incorporating a pooled internal standard (2003) Proteomics, 3, pp. 36-44
Yang, S., Zhu, H., Li, Y., Lin, H., Gabrielson, K., Trush, M.A., Diehl, A.M., Mitochondrial adaptations to obesity-related oxidant stress (2000) Arch. Biochem. Biophys., 378, pp. 259-268
Pessayre, D., Fromenty, B., NASH: a mitochondrial disease (2005) J. Hepathol., 42, pp. 928-940
Douette, P., Navet, R., Gerkens, P., de Pauw, E., Leprince, P., Sluse-Goffart, C.M., Sluse, F.E., Steatosis-induced proteomic changes in liver mitochodnria evidenced by two-dimensional differential in-gel electrophoresis (2005) J. Proteome Res., 4, pp. 2024-2031
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