alternative oxidase; yeast; mitoproteome; proteomics; two-dimensional differential in-gel electrophoresis
Abstract :
[en] The energy-dissipating alternative oxidase (AOX) from Hansenula anomala, was expressed in Saccharomyces cerevisiae. The recombinant AOX was functional. A comparative analysis by two-dimensional differential in-gel electrophoresis (2D-DIGE) of mitochondrial protein patterns found in wild-type and recombinant AOX strains was performed. 60 proteins exhibiting a significant difference in their abundance were identified. Interestingly, proteins implicated in major metabolic pathways such as Krebs cycle and amino acid biosynthesis were up-regulated. Surprisingly, an up-regulation of the respiratory-chain complex III was associated with a down-regulation of the ATP synthase complex.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Mathy, Grégory ; Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
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