Saccharomyces cerevisiae mitoproteome plasticity in response to recombinant alternative ubiquinol oxidase

[en] The energy-dissipating alternative oxidase (AOX) from Hansenula anomala, was expressed in Saccharomyces cerevisiae. The recombinant AOX was functional. A comparative analysis by two-dimensional differential in-gel electrophoresis (2D-DIGE) of mitochondrial protein patterns found in wild-type and recombinant AOX strains was performed. 60 proteins exhibiting a significant difference in their abundance were identified. Interestingly, proteins implicated in major metabolic pathways such as Krebs cycle and amino acid biosynthesis were up-regulated. Surprisingly, an up-regulation of the respiratory-chain complex III was associated with a down-regulation of the ATP synthase complex.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Mathy, Grégory ; Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire

Navet, Rachel ; Université de Liège - ULiège > GIGA - Formation

Gerkens, Pascal

Leprince, Pierre ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.

De Pauw, Edwin ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse

Sluse-Goffart, Claudine

Sluse, Francis ; Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire

Douette, Pierre ; Université de Liège - ULiège > Bioénergétique et physiologie cellulaire

Language :

English

Title :

Saccharomyces cerevisiae mitoproteome plasticity in response to recombinant alternative ubiquinol oxidase

Publication date :

February 2006

Journal title :

Journal of Proteome Research

ISSN :

1535-3893

eISSN :

1535-3907

Publisher :

Amer Chemical Soc, Washington, United States - Washington

Volume :

Issue :

Pages :

339-348

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

FRFC - Fonds de la Recherche Fondamentale Collective

Available on ORBi :

since 01 December 2008

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