Unpublished conference/Abstract (Scientific congresses and symposiums)
Study of the Bacillus subtilis PBP4* and RacX, YlmE racemases in relation with disassembly of biofilms
Vanden Broeck, Arnaud
2014IGBMC International PhD Programme interview session
 

Files


Full Text
Arnaud Vanden Broeck - Master work presentation.pptx
Author postprint (14.49 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Racemase; Biofilm; Bacillus
Abstract :
[en] Bacillus subtilis is a PGPR (Plant Growth Promoting Rhizobacterium) Gram positive bacterium and a model for studying the in vitro formation or disruption of biofilms. At the liquid/air interface of standing cultures, B. subtilis forms thick pellicles of limited lifetimes. Some D-amino acids have been reported among the factors playing a role in the disassembly of B. subtilis biofilms and ylmE or racX mutants (in which the racemases YlmE or RacX are absent) show a delay in pellicle disruption. The racX encoding gene is part of a bicistronic operon in which the second gene (pbpE) codes for a Penicillin-Binding Protein, the PBP4* whose function is not characterized in Bacillus. Our studies aim to produce, purify and characterize the role of the PBP4* and RacX, YlmE racemases in relation with the disassembly of biofilms. Biochemical and structural data have been obtained and the role of the three proteins has been partly solved. X-ray structures will permit us to create inhibitor molecules against Bacillus subtilis biofilm formation or disassembly.
Research center :
Centre for Protein Engineering
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Vanden Broeck, Arnaud ;  Université de Liège - ULiège > 2e an. master bioch. & biol. moléc. & cell., fin. appr.
Language :
English
Title :
Study of the Bacillus subtilis PBP4* and RacX, YlmE racemases in relation with disassembly of biofilms
Publication date :
01 April 2014
Number of pages :
35
Event name :
IGBMC International PhD Programme interview session
Event organizer :
IGBMC
Event place :
Strasbourg, France
Event date :
1st of April 2014
By request :
Yes
Audience :
International
Name of the research project :
Study of the formation and disassembly of Bacillus subtilis biofilm
Available on ORBi :
since 29 April 2014

Statistics


Number of views
185 (27 by ULiège)
Number of downloads
3 (3 by ULiège)

Bibliography


Similar publications



Contact ORBi