Near Infrared Hyperspectral Imaging; collagen; bone; Principal Components Analysis; Partial Least-Squares Discriminant Analysis; Holocene; Pleistocene; Paleolithic; Mesolithic; Neolithic
Abstract :
[en] The scope of this article is to propose an innovative method based on Near Infrared Hyperspectral Chemical Imaging (NIR-HCI) to rapidly and non-destructively evaluate the relative degree of collagen preservation in bones recovered from archaeological contexts. This preliminary study has allowed the evaluation of the potential of the method using bone samples from the Early Upper Palaeolithic, Mesolithic and Neolithic periods at the site of Trou Al'Wesse in Belgium. NIR-HCI, combined with chemometric tools, has identified specific spectral bands characteristic of collagen. A chemometric model has been built using Partial Least Squares Discriminant Analysis (PLS-DA) to identify bones with and without collagen. This enables the evaluation of the degree of collagen preservation and homogeneity in bones within and between different strata, which has direct implications for archaeological applications (e.g., taphonomic analyses, assemblage integrity) and sample selection for sub- sequent analyses requiring collagen. Two archaeological applications are presented: comparison between sub-layers in an Early Upper Palaeolithic unit, and evaluation of the range of variability in collagen preservation within a single Holocene stratum.
Disciplines :
Chemistry Archaeology
Author, co-author :
Vincke, Damien; Walloon Agricultural Research Centre (CRA-W), Gembloux > Valorisation of Agricultural Products > Product Quality
Miller, Rebecca ; Université de Liège - ULiège > Département des sciences historiques > Archéologie préhistorique
Stassart, Edith ; Université de Liège - ULiège > Département des sciences historiques > Archéologie préhistorique
Otte, Marcel ; Université de Liège - ULiège > Département des sciences historiques > Archéologie préhistorique
Dardenne, Pierre; Walloon Agricultural Research Centre (CRA-W), Gembloux > Valorisation of Agricultural Products > Product Quality
Collins, Matthew; University of York (York, UK) > Archaeology
Wilkinson, Keith; University of Winchester (Winchester, UK) > Archaeology
Stewart, John; Bournemouth University (Bournemouth, UK) > Science and Technology
Baeten, Vincent; Walloon Agricultural Research Centre (CRA-W), Gembloux > Valorisation of Agricultural Products > Product Quality
Fernandez Pierna, Juan; Walloon Agricultural Research Centre (CRA-W), Gembloux > Valorisation of Agricultural Products > Product Quality
Language :
English
Title :
Analysis of collagen preservation in bones recovered in archaeological contexts using NIR Hyperspectral Imaging
Publication date :
2014
Journal title :
Talanta
ISSN :
0039-9140
eISSN :
1873-3573
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
125
Pages :
181-188
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique Service public de Wallonie : Direction générale opérationnelle de l'aménagement du territoire, du logement, du patrimoine et de l'énergie - DG04
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