Article (Scientific journals)
A highly stable Yarrowia lipolytica lipase formulation for the treatment of pancreatic exocrine insufficiency
Turki, S.; Mrabet, G.; Jabloun, Zeineb et al.
2010In Biotechnology and Applied Biochemistry, 57 (4), p. 139-149
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Keywords :
Bile salts; Digestive proteases; In vitro stability; Lipase; PH; Yarrowia lipolytica; Lipase activity; Milk powder; Physiological condition
Abstract :
[en] Yarrowia lipolytica lipase has been assumed to be a good candidate for the treatment of fat malabsorption in patients with pancreatic insufficiency. Nevertheless, no systematic studies on its stability under physiological conditions pertaining to the human GI (gastrointestinal) tract have been published. Stability of various Y. lipolytica lipase powder formulations at various physiological pH values as well as the effect of digestive proteases and bile salts on enzyme activity were investigated. Results were compared with those obtained from another competing fungal lipase sourced fromCandida rugosa. Among the studied formulations, Y. lipolytica lipase stabilized with gum arabic and skimmed milk powder was the most promising powder formulation. Under acidic conditions (pH 3-5), this formulation showed higher stability than those observed with the other Y. lipolytica lipase formulations and C. rugosa lipase. In addition, in the presence of gum arabic and skimmed milk powder as additives, Y. lipolytica lipase exhibited markedly higher resistance to pepsin, trypsin and chymotrypsin actions. Resistance to proteolytic degradation by digestive proteases was also by far higher than that observed with C. rugosa lipase. Similar behaviour was, however, observed when these two fungal lipases were incubated with increased concentrations of bile salts. Residual lipase activity of both fungal lipases showed a slight decrease in NaTDC (sodium taurodeoxycholate) concentration above 4 mM. Consequently, Y. lipolytica lipase formulated with gum arabic and milk powder seemed to have great potential for use as a therapeutic tool for patients with pancreatic insufficiency. © 2010 Portland Press Limited.
Disciplines :
Microbiology
Author, co-author :
Turki, S.;  Fermentation Unit, Institut Pasteur de Tunis, 1002 Tunis Belvédère, Tunisia
Mrabet, G.;  Fermentation Unit, Institut Pasteur de Tunis, 1002 Tunis Belvédère, Tunisia
Jabloun, Zeineb;  Fermentation Unit, Institut Pasteur de Tunis, 1002 Tunis Belvédère, Tunisia
Destain, Jacqueline ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Kallel, H.;  Fermentation Unit, Institut Pasteur de Tunis, 1002 Tunis Belvédère, Tunisia
Language :
English
Title :
A highly stable Yarrowia lipolytica lipase formulation for the treatment of pancreatic exocrine insufficiency
Publication date :
2010
Journal title :
Biotechnology and Applied Biochemistry
ISSN :
0885-4513
eISSN :
1470-8744
Publisher :
Wiley-Blackwell, United States
Volume :
57
Issue :
4
Pages :
139-149
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 March 2014

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