Article (Scientific journals)
A multiple-level study of metal tolerance in Salix fragilis and Salix aurita clones
Evlard, Aricia; Sergeant, Kjell; Printz, Bruno et al.
2014In Journal of Proteomics, 101C, p. 113-129
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Keywords :
willow; metal trace element; proteomics
Abstract :
[en] The response of two willow clones (Salix fragilis (Sf) and S. aurita (Sa)) to the presence of metals (Zn, Cu, Cd, Ni) was studied. Rooted cuttings were planted in control and contaminated soil. After 100 days, different parameters (biomass, chlorophyll fluorescence (Fv/Fm), pigment and sugar concentrations, electrolyte leakage and proteome-level changes) were analyzed. The growth of Sa was not influenced by metals whereas Sf produced significantly less biomass when exposed to the pollutants. Furthermore, although Sa did not show a growth reduction in the presence of metals, the overall view of the physiological results among others the changes in the accumulation of sugars and pigments indicated that metals had a more severe impact on this clone. The response at the proteome level confirmed these observations. The growth reduction and the proteomic changes in Sf indicate that this clone adjusts its metabolism to maintain cellular homeostasis. Sa on the contrary maintains growth but the physiological and proteomics data suggests that this can only be done at the cost of cellular deregulation. Therefore high biomass is not linked with a good tolerance strategy. In a long-term study the survival of Sa might be compromised making it a poorer candidate for phytoremediation efforts.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Evlard, Aricia  ;  Université de Liège - ULiège > Sciences agronomiques > Laboratoire de Toxicologie environnementale
Sergeant, Kjell ;  Centre de Recherche Public — Gabriel Lippmann > Environment and Agrobiotechnologies
Printz, Bruno;  Centre de Recherche Public — Gabriel Lippmann > Environment and Agrobiotechnologies
Guignard, Cédric;  Centre de Recherche Public — Gabriel Lippmann
Renaut, Jenny;  Centre de Recherche Public — Gabriel Lippmann
Campanella, Bruno
Paul, Roger ;  Université de Liège - ULiège > Sciences agronomiques > Laboratoire de Toxicologie environnementale
Hausman, Jean-Francois;  Centre de Recherche Public — Gabriel Lippmann
 These authors have contributed equally to this work.
Language :
English
Title :
A multiple-level study of metal tolerance in Salix fragilis and Salix aurita clones
Publication date :
14 April 2014
Journal title :
Journal of Proteomics
ISSN :
1874-3919
eISSN :
1876-7737
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
101C
Pages :
113-129
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 March 2014

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