BcsTx3 is a founder of a novel sea anemone toxin family of potassium channel blocker

[en] Sea anemone venoms have become a rich source of peptide toxins which are invaluable tools for studying the structure and functions of ion channels. In this work, BcsTx3, a toxin found in the venom of a Bunodosoma caissarum (population captured at the Saint Peter and Saint Paul Archipelago, Brazil) was purified and biochemically and pharmacologically characterized. The pharmacological effects were studied on 12 different subtypes of voltage-gated potassium channels (KV1.1–KV1.6; KV2.1; KV3.1; KV4.2; KV4.3; hERG and Shaker IR) and three cloned voltagegated sodium channel isoforms (NaV1.2, NaV1.4 and BgNaV1.1) expressed in Xenopus laevis oocytes. BcsTx3 shows a high affinity for Drosophila Shaker IR channels over rKv1.2, hKv1.3 and rKv1.6, and is not active on NaV channels. Biochemical characterization reveals that BcsTx3 is a 50 amino acid peptide crosslinked by four disulfide bridges, and sequence comparison allowed BcsTx3 to be classified as a novel type of sea anemone toxin acting on KV channels. Moreover, putative toxins homologous to BcsTx3 from two additional actiniarian species suggest an ancient origin of this newly discovered toxin family.

Disciplines :

Chemistry

Author, co-author :

Orts, Diego

Moran, Yehu

Cologna, Camila

Peigneur, Steeve

Bruno, Madio

Praher, Daniela

Quinton, Loïc ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Bicudo, José

Tytgat, jan

de Freitas, José

Language :

English

Title :

BcsTx3 is a founder of a novel sea anemone toxin family of potassium channel blocker

Publication date :

2013

Journal title :

FEBS Journal

ISSN :

1742-464X

eISSN :

1742-4658

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

280

Pages :

4839-4852

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 12 March 2014

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