Inactivation of genes coding for mitochondrial Nd7 and Nd9 complex I subunits in Chlamydomonas reinhardtii. Impact of complex I loss on respiration and energetic metabolism.
[en] In Chlamydomonas, unlike in flowering plants, genes coding for Nd7 (NAD7/49kDa) and Nd9 (NAD9/30kDa) core subunits of mitochondrial respiratory-chain complex I are nucleus-encoded. Both genes possess all the features that facilitate their expression and proper import of the polypeptides in mitochondria. By inactivating their expression by RNA interference or insertional mutagenesis, we show that both subunits are required for complex I assembly and activity. Inactivation of complex I impairs the cell growth rate, reduces the respiratory rate, leads to lower intracellular ROS production and lower expression of ROS scavenging enzymes, and is associated to a diminished capacity to concentrate CO2 without compromising photosynthetic capacity.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Massoz, Simon; Université de Liège - ULiège > Département des sciences de la vie > Génétique des microorganismes
Larosa, Véronique ; Université de Liège - ULiège > Département des sciences de la vie > Génétique des microorganismes
Plancke, Charlotte; Université de Liège - ULiège > Département des sciences de la vie > Génétique des microorganismes
Lapaille, Marie; Université de Liège - ULiège > Département des sciences de la vie > Génétique des microorganismes
Bailleul, Benjamin ; Université de Liège - ULiège > Département des sciences de la vie > Génétique des microorganismes
Pirotte, Dorothée ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques
Radoux, Michele; Université de Liège - ULiège > Département des sciences de la vie > Génétique des microorganismes
Leprince, Pierre ; Université de Liège - ULiège > GIGA - Neurosciences
Coosemans, Nadine ; Université de Liège - ULiège > Département des sciences de la vie > Génétiques des microorganismes
Matagne, René-Fernand ; Université de Liège - ULiège > Relations académiques et scientifiques (Sciences)
Remacle, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Génétique des microorganismes
Cardol, Pierre ; Université de Liège - ULiège > Département des sciences de la vie > Génétique des microorganismes
Language :
English
Title :
Inactivation of genes coding for mitochondrial Nd7 and Nd9 complex I subunits in Chlamydomonas reinhardtii. Impact of complex I loss on respiration and energetic metabolism.
Publication date :
04 December 2013
Journal title :
Mitochondrion
ISSN :
1567-7249
eISSN :
1872-8278
Publisher :
Elsevier, Netherlands
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2013 (c) Elsevier B.V. and Mitochondria Research Society. All rights reserved. Published by Elsevier B.V. All rights reserved.
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