[en] The aim of this study was to describe the embryos abortion process and the inheritance of the embryos abortion trait in Phaseolus vulgaris plants deficient in seed development. These plants were isolated within the second generation of an ethyl methanesulfonate (EMS) TILLING population of P. vulgaris cv. 'BAT93'. Mutant embryos show abnormalities mainly in suspensors, shoot apical meristem (SAM) and cotylédons from the globular to the cotyledon stages and abort before maturity compared to those observed in wild-types samples. Mutant embryos show also hyperhydricity and contain low amount of chlorophyll. Genetic analyses of F1, F2 and F3 populations from the crosses carried out between the mutagenized plants with aborting embryos and the wild-type plants indicated that the embryo abortion phenotype is maternally inherited and controlled by a single recessive gene. These Phaseolus mutant plants with aborting embryos constitute a valuable material for plant embryogenesis studies. [fr] Le but de cette étude était de décrire le processus d'avortement des embryons et d'étudier la transmission du caractère d'avortement des embryons chez les plantes de Phaseolus vulgaris déficientes dans le développement normal de la graine. Ces plantes ont été identifiées au sein de la deuxième génération d'une population TILLING de P. vulgaris cv. 'BAT93' obtenue par mutagénèse au méthanesulfonate (EMS). Les embryons mutants présentent des anomalies au niveau du suspenseur, du méristème apical caulinaire et des cotylédons, du stade globulaire jusqu'au stade cotylédonaire et avortent avant la maturité, comparé à ce qui se passe chez les embryons normaux. Les embryons mutants montrent également une hyperhydricité et contiennent une quantité de faible de chlorophylle. Les analyses génétiques des populations F1, F2 et F3 issues des croisement effectués entre les plantes mutantes avec des embryons qui avortent et les plantes de type sauvage ont indiqué que le phénotype avortement des embryons est à hérédité maternelle et contrôlé par un couple d'allèles récessifs. Ces plantes mutantes de Phaseolus constituent un matériel précieux pour les études sur l'embryogenèse des plantes.
Disciplines :
Agriculture & agronomy
Author, co-author :
Silué, Souleymane; Université de Liège - Gembloux Agro-Bio Tech ; Peleforo Gon Coulibaly University of Korhogo ; Walloon Agricultural Research Centre
Diarrasouba, Nafan; Peleforo Gon Coulibaly University of Korhogo > Biological Sciences Department
Fofana, Inza Jesus; Peleforo Gon Coulibaly University of Korhogo > Biological Sciences Department
Muhovski, Yordan; Walloon Agricultural Research Centre > Department of Life Sciences > Unit of Breeding and Biodiversity
Toussaint, André ; Université de Liège - ULiège > Sciences agronomiques > Phytotechnie tropicale et horticulture
Mergeai, Guy ; Université de Liège - ULiège > Sciences agronomiques > Phytotechnie tropicale et horticulture
Jacquemin, Jean-Marie; Walloon Agricultural Research Centre > Department of Life Sciences > Unit of Breeding and Biodiversity
Baudoin, Jean-Pierre ; Université de Liège - ULiège > Sciences agronomiques > Phytotechnie tropicale et horticulture
Language :
English
Title :
Description of Phaseolus vulgaris L. aborting embryos from ethyl methanesulfonate (EMS) mutagenized plants
Alternative titles :
[en] Description des embryons de Phaseolus vulgaris en cours d'avortement issus de plantes mutagénisées au méthanesulfonate d'éthyle (EMS)
Publication date :
2013
Journal title :
Biotechnologie, Agronomie, Société et Environnement
ISSN :
1370-6233
eISSN :
1780-4507
Publisher :
Presses Agronomiques de Gembloux, Gembloux, Belgium
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