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Measurement of cerebrospinal fluid albumin in healthy dogs
Ramery, Eve; Girod, Maud; Allerton, Fergus et al.
2013In Veterinary Clinical Pathology, p. 1-29
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Keywords :
Albumin; CSF; Dog
Abstract :
[en] Background: Measurement of CSF albumin aids diagnosis in human medicine but technical difficulties related to its low CSF concentration prohibit its routine use in veterinary medicine. High-resolution electrophoresis (HRE) has been described but often results in non-interpretable integration profiles preventing albumin determination. Fraction quantification using HRE may be more precise after concentration (cHRE) using a membrane microconcentrator technique but has not been evaluated in CSF with total protein levels below 20mg/dL. Immunoturbidimetry is routinely used for human CSF albumin measurement and was recently applied on canine samples with encouraging results. Objective: The purpose of this study was to compare HRE (including the use of a concentration step) and immunoturbidimetric assay for the measurement of albumin levels in normal canine CSF. Methods: 30 CSF specimens from 15 healthy dogs were evaluated. CSF total protein was measured by the pyrogallol red methoda and CSF albumin was determined by HREb (n=15), cHREc (n=30) and immunoturbidimetric assayd (n=30). Validation of the human immunoturbidimetric assay was performed using a purified canine albumin standarde. Results: Mean CSF total protein was 17.5 (range 7-39) mg/dL. HRE integration profiles were non-interpretable in all unconcentrated specimens. However, clear distinction of the major protein fractions was achieved for all cHRE specimens. CSF albumin levels were measureable in 29/30 specimens using immunoturbidimetry. Excellent correlation (Pearson r=0.92, p<0.001) was found between the two techniques. Conclusion: Immunoturbidimetry and cHRE may be used for routine measurement of CSF albumin.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ramery, Eve ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Biochimie
Girod, Maud ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
Allerton, Fergus
Gommeren, Kris  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
DeMarchin, Jerôme
Peeters, Dominique ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Médecine interne des animaux de compagnie
Language :
English
Title :
Measurement of cerebrospinal fluid albumin in healthy dogs
Publication date :
2013
Event name :
15th annual congress of the European Society and College of Veterinary Clinical Pathology ESVCP/ECVCP
Event date :
November 6-9, 2013
Audience :
International
Journal title :
Veterinary Clinical Pathology
ISSN :
0275-6382
Publisher :
American Society for Veterinary Clinical Pathology, Baton Rouge, United States - Louisiana
Pages :
1-29
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 February 2014

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