Reference : Characterization of an unusual thyroid response unit in the promoter of the human pla...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/16348
Characterization of an unusual thyroid response unit in the promoter of the human placental lactogen gene
English
Voz, Marianne mailto [Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Peers, Bernard mailto [Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Belayew, Alexandra [> > > >]
Martial, Joseph mailto [Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
1991
Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology
266
20
13397-404
Yes (verified by ORBi)
International
0021-9258
1083-351X
Baltimore
MD
[en] Animals ; Base Sequence ; Binding Sites ; Cell Line ; Cell Nucleus/physiology ; Chloramphenicol O-Acetyltransferase/genetics/metabolism ; Chromosome Deletion ; Hela Cells/physiology ; Humans ; Molecular Sequence Data ; Nucleotide Mapping ; Oligonucleotide Probes ; Placental Lactogen/*genetics ; Plasmids ; Promoter Regions, Genetic/*drug effects ; Rats ; Sequence Homology, Nucleic Acid ; Transfection ; Triiodothyronine/*pharmacology
[en] The human placental lactogen B (hCS-B) promoter activity is strongly stimulated by thyroid hormones in the rat pituitary GC cell line. The minimal DNA sequence required for stimulation, as determined by transfection with 5' and 3' deletion mutants, spans 67 base pairs, from coordinate -97 to -31. DNase I footprinting experiments show that this thyroid response unit includes two adjacent binding sites: one for the thyroid receptor (-67/-41), the other for the pituitary-specific factor GHF1 (-95/-68). Neither region alone is sufficient to confer thyroid responsiveness. The thyroid receptor binding element (TBE) does not contain any repeats or palindromes but is composed of two different domains, one of which is very similar to the half-palindromic motif described by Glass et al. (Glass, C.K., Holloway, J.M., Devary, O.L., and Rosenfeld, M.G. (1988) Cell 54, 313-323). The other is very rich in purine. The normal human growth hormone (hGH-N) promoter, which is 94% similar to the hCS-B promoter, differs from its hCS-B counterpart precisely in this TBE. This difference may explain the opposite 3,5,3'-triiodothyronine (T3) regulation of these two genes.
http://hdl.handle.net/2268/16348
http://www.jbc.org/cgi/reprint/266/20/13397
1991/07/15

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