Pseudomonas aeruginosa displays an epidemic population structure.

Alleles; Bacterial Outer Membrane Proteins/genetics; Base Sequence; DNA, Bacterial/analysis/genetics; Databases, Nucleic Acid; Genes, Bacterial/genetics; Molecular Sequence Data; Pseudomonas Infections/epidemiology/microbiology; Pseudomonas aeruginosa/classification/genetics; Serotyping

Abstract :

[en] Bacteria can have population structures ranging from the fully sexual to the highly clonal. Despite numerous studies, the population structure of Pseudomonas aeruginosa is still somewhat contentious. We used a polyphasic approach in order to shed new light on this issue. A data set consisting of three outer membrane (lipo)protein gene sequences (oprI, oprL and oprD), a DNA-based fingerprint (amplified fragment length polymorphism), serotype and pyoverdine type of 73 P. aeruginosa clinical and environmental isolates, collected across the world, was analysed using biological data analysis software. We observed a clear mosaicism in the results, non-congruence between results of different typing methods and a microscale mosaic structure in the oprD gene. Hence, in this network, we also observed some clonal complexes characterized by an almost identical data set. The most recent clones exhibited serotypes O1, 6, 11 and 12. No obvious correlation was observed between these dominant clones and habitat or, with the exception of some recent clones, geographical origin. Our results are consistent with, and even clarify, some seemingly contradictory results in earlier epidemiological studies. Therefore, we suggest an epidemic population structure for P. aeruginosa, comparable with that of Neisseria meningitidis, a superficially clonal structure with frequent recombinations, in which occasionally highly successful epidemic clones arise.

Disciplines :

Biochemistry, biophysics & molecular biology
Microbiology

Author, co-author :

Pirnay, Jean-Paul

De Vos, Daniel

Cochez, Christel

Bilocq, Florence

Vanderkelen, Alain

Zizi, Martin

Ghysels, Bart ; Vrije Universiteit Brussel - VUB > Laboratory of Microbial Interactions

Cornelis, Pierre

Language :

English

Title :

Pseudomonas aeruginosa displays an epidemic population structure.

Publication date :

2002

Journal title :

Environmental Microbiology

ISSN :

1462-2912

eISSN :

1462-2920

Publisher :

Wiley, Oxford, United Kingdom

Volume :

Issue :

Pages :

898-911

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 29 January 2014

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106

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