Article (Scientific journals)
FpvB, an alternative type I ferripyoverdine receptor of Pseudomonas aeruginosa.
Ghysels, Bart; Dieu, Bui Thi Min; Beatson, Scott A. et al.
2004In Microbiology, 150 (Pt 6), p. 1671-80
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Keywords :
Amino Acid Sequence; Bacterial Outer Membrane Proteins/chemistry/genetics/metabolism; Bacterial Proteins/genetics/metabolism; Base Sequence; Gene Expression Regulation, Bacterial; Iron/metabolism; Molecular Sequence Data; Mutation; Oligonucleotide Array Sequence Analysis; Oligopeptides; Pigments, Biological/metabolism; Pseudomonas aeruginosa/genetics/growth & development/metabolism; Sequence Analysis, DNA; Siderophores/metabolism
Abstract :
[en] Under conditions of iron limitation, Pseudomonas aeruginosa secretes a high-affinity siderophore pyoverdine to scavenge Fe(III) in the extracellular environment and shuttle it into the cell. Uptake of the pyoverdine-Fe(III) complex is mediated by a specific outer-membrane receptor protein, FpvA (ferripyoverdine receptor). Three P. aeruginosa siderovars can be distinguished, each producing a different pyoverdine (type I-III) and a cognate FpvA receptor. Growth of an fpvA mutant of P. aeruginosa PAO1 (type I) under iron-limiting conditions can still be stimulated by its cognate pyoverdine, suggesting the presence of an alternative uptake route for type I ferripyoverdine. In silico analysis of the PAO1 genome revealed that the product of gene PA4168 has a high similarity with FpvA. Inactivation of PA4168 (termed fpvB) in an fpvA mutant totally abolished the capacity to utilize type I pyoverdine. The expression of fpvB is induced by iron limitation in Casamino acids (CAA) and in M9-glucose medium, but, unlike fpvA, not in a complex deferrated medium containing glycerol as carbon source. The fpvB gene was also detected in other P. aeruginosa isolates, including strains producing type II and type III pyoverdines. Inactivation of the fpvB homologues in these strains impaired their capacity to utilize type I ferripyoverdine as a source of iron. Accordingly, introduction of fpvB in trans restored the capacity to utilize type I ferripyoverdine.
Disciplines :
Microbiology
Biochemistry, biophysics & molecular biology
Author, co-author :
Ghysels, Bart ;  Vrije Universiteit Brussel - VUB > Laboratory of Microbial Interactions
Dieu, Bui Thi Min
Beatson, Scott A.
Pirnay, Jean-Paul
Ochsner, Urs A.
Vasil, Michael L.
Cornelis, Pierre
Language :
English
Title :
FpvB, an alternative type I ferripyoverdine receptor of Pseudomonas aeruginosa.
Publication date :
2004
Journal title :
Microbiology
ISSN :
1350-0872
eISSN :
1465-2080
Publisher :
Society for General Microbiology, United Kingdom
Volume :
150
Issue :
Pt 6
Pages :
1671-80
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 29 January 2014

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