Control of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii.

Tolleter, Dimitri; Ghysels, Bart; Alric, Jean; Petroutsos, Dimitris; Tolstygina, Irina; Krawietz, Danuta; Happe, Thomas; Auroy, Pascaline; Adriano, Jean-Marc; Beyly, Audrey; Cuine, Stephan; Plet, Julie; Reiter, Ilja M.; Genty, Bernard; Cournac, Laurent; Hippler, Michael; Peltier, Gilles

doi:10.1105/tpc.111.086876

Article (Scientific journals)

Control of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii.

Tolleter, Dimitri; Ghysels, Bart; Alric, Jean et al.

2011 • In Plant Cell, 23 (7), p. 2619-30

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https://hdl.handle.net/2268/162361

DOI
10.1105/tpc.111.086876

PubMed
21764992

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Keywords :

Aerobiosis; Anaerobiosis; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone/pharmacology; Chlamydomonas reinhardtii/cytology/genetics/metabolism; Electron Transport/drug effects/physiology; Electrons; Genetic Complementation Test; Hydrogen/metabolism; Hydrogenase/metabolism; Light; Membrane Proteins/genetics/metabolism; Oxidation-Reduction; Oxygen/metabolism; Photosynthesis/drug effects/physiology; Photosystem I Protein Complex/drug effects/genetics/metabolism; Plant Proteins/genetics/metabolism; Plants, Genetically Modified; Proton Ionophores/pharmacology; Protons; Sulfur/metabolism

Abstract :

[en] Hydrogen photoproduction by eukaryotic microalgae results from a connection between the photosynthetic electron transport chain and a plastidial hydrogenase. Algal H(2) production is a transitory phenomenon under most natural conditions, often viewed as a safety valve protecting the photosynthetic electron transport chain from overreduction. From the colony screening of an insertion mutant library of the unicellular green alga Chlamydomonas reinhardtii based on the analysis of dark-light chlorophyll fluorescence transients, we isolated a mutant impaired in cyclic electron flow around photosystem I (CEF) due to a defect in the Proton Gradient Regulation Like1 (PGRL1) protein. Under aerobiosis, nonphotochemical quenching of fluorescence (NPQ) is strongly decreased in pgrl1. Under anaerobiosis, H(2) photoproduction is strongly enhanced in the pgrl1 mutant, both during short-term and long-term measurements (in conditions of sulfur deprivation). Based on the light dependence of NPQ and hydrogen production, as well as on the enhanced hydrogen production observed in the wild-type strain in the presence of the uncoupling agent carbonyl cyanide p-trifluoromethoxyphenylhydrazone, we conclude that the proton gradient generated by CEF provokes a strong inhibition of electron supply to the hydrogenase in the wild-type strain, which is released in the pgrl1 mutant. Regulation of the trans-thylakoidal proton gradient by monitoring pgrl1 expression opens new perspectives toward reprogramming the cellular metabolism of microalgae for enhanced H(2) production.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Tolleter, Dimitri ^✱

Ghysels, Bart ^✱; Commissariat à l'Energie Atomique (Cadarache) - CEA > Laboratoire de Bioe´ nerge´tique et Biotechnologie des Bacte´ ries et Microalgues

Alric, Jean

Petroutsos, Dimitris

Tolstygina, Irina

Krawietz, Danuta

Happe, Thomas

Auroy, Pascaline

Adriano, Jean-Marc

Beyly, Audrey

More authors (7 more)

^✱ These authors have contributed equally to this work.

Language :

English

Title :

Control of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii.

Publication date :

2011

Journal title :

Plant Cell

ISSN :

1040-4651

eISSN :

1532-298X

Publisher :

American Society of Plant Biologists, United States - Maryland

Volume :

Issue :

Pages :

2619-30

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 29 January 2014

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