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Innovative analytical strategy using ion-mobility for structural or functional selenium isomers identification by ion mobility spectrometry
Far, Johann; Kune, Christopher; Lobinski, Ryszard et al.
2013Metallomics 2013
 

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Keywords :
Selenium speciation; Ion Mobility Mass Spectrometry; Isomer identification and quantification
Abstract :
[en] Selenium (Se) is a trace element which is both essential and toxic depending on its concentration and its chemical form. Se-rich yeast is one of the most popular Se source for supplementation. The classical method of speciation is related to multidimensional liquid chromatography (LC) hyphenated to mass spectrometry (MS) Recent advances in Se speciation led to greatly improve the Se speciation in these samples but isomers identification and quantification remain challenging. This work focuses on the elaboration of an innovative analytical strategy for the detection and the structural elucidation of isobaric selenium compounds present in Se-rich yeast. A specific complex formation agent acts as a chemical probe for the detection of chemical function. The addition of a complexing agent can improve the discrimination between structural or functional Se isomers using ion mobility techniques as Ion Mobility Spectrometry (IMS) by increasing the molecular weight (i.e. the m/z ratio in MS) and the collision cross section of a target ion after selective complexation. This Ion Mobility orthogonal separation improves the structural elucidation. Crown ethers used as shifting agents can specifically form complexes with primary amines. The addition of crown ether to different low molecular weight fractions obtained by multidimensional LC of a water extract from Se-rich yeast permitted to detect Se isomers and confirmed their structure using IMS.
Disciplines :
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Far, Johann  ;  Université de Liège - ULiège > Center for Analytical Research and Technology (CART)
Kune, Christopher  ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Lobinski, Ryszard;  Université de Pau et des Pays de L'adour > CNRS/UPPA, Laboratoire de Chimie Analytique Bio-inorganique et Environnement (LCABIE)
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Eppe, Gauthier  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Language :
English
Title :
Innovative analytical strategy using ion-mobility for structural or functional selenium isomers identification by ion mobility spectrometry
Publication date :
July 2013
Number of pages :
A0
Event name :
Metallomics 2013
Event place :
Oviedo, Spain
Event date :
du 8 au 11 Juillet 2013
Audience :
International
Funders :
ULiège - Université de Liège [BE]
Available on ORBi :
since 28 January 2014

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