Psychrophilic Enzymes: A Thermodynamic Challenge

[en] Psychrophilic microorganisms, hosts of permanently cold habitats, produce enzymes which are adapted to work at low temperatures. When compared to their mesophilic counterparts, these enzymes display a higher catalytic efficiency over a temperature range of roughly 0-30 degrees C and a high thermosensitivity. The molecular characteristics of cold enzymes originating from Antarctic bacteria have been approached through protein modelling and X-ray crystallography. The deduced three-dimensional structures of cold alpha-amylase, beta-lactamase, lipase and subtilisin have been compared to their mesophilic homologs. It appears that the molecular adaptation resides in a weakening of the intramolecular interactions, and in some cases in an increase of the interaction with the solvent, leading to more flexible molecular edifices capable of performing catalysis at a lower energy cost.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Gerday, Charles ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)

Aittaleb, Mohamed

Arpigny, Jean Louis

Baise, Etienne ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques

Chessa, Jean-Pierre

Garsoux, Geneviève

Petrescu, Ioan

Feller, Georges ; Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie

Language :

English

Title :

Psychrophilic Enzymes: A Thermodynamic Challenge

Publication date :

17 October 1997

Journal title :

Biochimica et Biophysica Acta

ISSN :

0006-3002

eISSN :

1878-2434

Publisher :

Elsevier, Amsterdam, Netherlands

Volume :

1342

Issue :

Pages :

119-31

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 January 2010

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256

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