Reference : Proteomic study of lumbar spinal cord after quadricipital eccentric exercise
Scientific congresses and symposiums : Poster
Human health sciences : Neurology
http://hdl.handle.net/2268/162142
Proteomic study of lumbar spinal cord after quadricipital eccentric exercise
English
[fr] Etude protéomique de la moelle épinière lombaire après un exercice eccentrique quadricipital
Lacrosse, Zoé mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine >]
Hody, Stéphanie mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine >]
Leprince, Pierre mailto [Université de Liège - ULiège > > GIGA - Neurosciences >]
Croisier, Jean-Louis mailto [Université de Liège - ULiège > Département des sciences de la motricité > Kinésithérapie générale et réadaptation >]
Rogister, Bernard mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine >]
12-Sep-2013
A0
No
No
International
Euron PhD Day
du 12 septembre 2013 au 13 septembre 2013
Ulg Giga-Neurosciences
Liège
Belgium
[en] Lumbar spinal cord ; Eccentric exercise ; Proteomic
[en] Eccentric muscle contractions are characterized by an increase of muscle tension as it lengthens (slowering movements). Unaccustomed or intense eccentric exercise causes “Delayed-Onset Muscle Soreness” (DOMS). DOMS include muscle pain that appears 24 to 72 hours after exercise, but also stiffness, edema and muscle proteins release in plasma as a hallmark of muscle fibers injuries. The only systematic intervention that brings a muscle protection against DOMS is to realize submaximal eccentric contractions with a progressively increased intensity. The mechanism of this protection, called the “Repeated Bout Effect” (RBE), is not understood. However, it is likely explained by cellular, mechanical and neural theories [Scand.J.Med.&Sci.Sports, 13, 88, 2003]. The objective of this study is to better understand which neural signal is released in the muscle synapse and which brings protection by RBE.
Male adult mice (C57BL6) were randomly divided into downhill running (DHR), uphill running (UHR) and untrained control (CONT) groups (n=4/group). DHR group is characterized by eccentric contractions of the quadriceps while UHR is concerned by concentric contractions. Running groups performed a warm-up of ten minutes followed by an interval exercise on an inclined treadmill at a velocity of 20cm/s. The latter consisted of running 18 bouts of 5 minutes interspersed with a 2 minutes rest. Lumbar spinal cord was dissected 24h after the race. Nuclear and cytoplasmic proteins were separately extracted and subjected to a 2D-DIGE analysis coupled with mass spectrometry.
We do not observe any cytoplasmic protein modification while in the nuclear extract, seven spots were more abundant in eccentric group and four in concentric group in comparison with control group. The mass spectrometry of these proteins reveals that they are implicated in axoplasmic transport.
At 24 hours, too few proteins modifications were detected in lumbar spinal cord, maybe as a consequence of a too short period between race and euthanasia. Implication of axoplasmic transport comforts our starting hypothesis that nervous system is able to protect muscle during the RBE by a synthesis and then a synaptic release of molecules modifying the muscle physiology.
Giga-Neurosciences
Role of nervous system in muscle typology remodeling induce by eccentric exercise
Researchers
http://hdl.handle.net/2268/162142

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