Expression of Psychrophilic Genes in Mesophilic Hosts: Assessment of the Folding State of a Recombinant Alpha-Amylase

[en] Alpha-Amylase from the antarctic psychrophile Altermonas haloplanktis is synthesized at 0 +/- 2 degrees C by the wild strain. This heat-labile alpha-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the described expression system, a compromise between enzyme stability and E. coli growth rate is reached at 18 degrees C.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Feller, Georges ; Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie

Le Bussy, O.

Gerday, Charles ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)

Language :

English

Title :

Expression of Psychrophilic Genes in Mesophilic Hosts: Assessment of the Folding State of a Recombinant Alpha-Amylase

Publication date :

March 1998

Journal title :

Applied and Environmental Microbiology

ISSN :

0099-2240

eISSN :

1098-5336

Publisher :

American Society for Microbiology, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

1163-5

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 January 2010

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Bibliography

Davail, S., G. Feller, E. Narinx, and C. Gerday. 1992. Sequence of the subtilisin-encoding gene from an antarctic psychrotroph Bacillus TA41. Gene 119:143-144.
Feller, G., T. Lonhienne, C. Deroanne, C. Libioulle, J. Van Beeumen, and C. Gerday. 1992. Purification, characterization and nucleotide sequence of the thermolabile α-amylase from the antarctic psychrotroph Alteromonas haloplanctis A23. J. Biol. Chem. 267:5217-5221.
Feller, G., E. Narinx, J. L. Arpigny, M. Aittaleb, E. Baise, S. Genicot, and C. Gerday. 1996. Enzymes from psychrophilic organisms. FEMS Microbiol. Rev. 18:189-202.
Feller, G., F. Payan, F. Theys, M. Qian, R. Haser, and C. Gerday. 1994. Stability and structural analysis of α-amylase from the antarctic psychrophile Alteromonas haloplanctis A23. Eur. J. Biochem. 222:441-447.
Feller, G., M. Thiry, J. L. Arpigny, and C. Gerday. 1991. Cloning and expression in Escherichia coli of three lipase-encoding genes from the psychrotrophic antarctic strain Moraxella TA144. Gene 102:111-115.
Gounot, A. M. 1991. Bacterial life at low temperature: physiological aspects and biotechnological implications. J. Appl. Bacteriol. 71:386-397.
Kobori, H., C. W. Sullivan, and H. Shizuya. 1984. Heat-labile alkaline phosphatase from antarctic bacteria: rapid 5′ end-labeling of nucleic acids. Proc. Natl. Acad. Sci. USA 81:6691-6695.
Margesin, R., and F. Schinner. 1994. Properties of cold-adapted microorganisms and their potential role in biotechnology. J. Biotechnol. 33:1-14.
Narinx, E., S. Davail, G. Feller, and C. Gerday. 1992. Nucleotide and derived amino acid sequence of the subtilisin from the antarctic psychrotroph Bacillus TA39. Biochim. Biophys. Acta 1131:111-113.
Pace, C. N., B. A. Shirley, and J. A. Thomson. 1989. Measuring the conformational stability of a protein, p. 311-330. In T. E. Creighton (ed.), Protein structure, a practical approach. IRL Press, Oxford, England.
Russel, N. J. 1992. Physiology and molecular biology of psychrophilic microorganisms, p. 203-224. In R. A. Herbert and R. J. Sharp (ed.), Molecular biology and biotechnology of extremophiles. Blackie, London, England.