[en] Alpha-Amylase from the antarctic psychrophile Altermonas haloplanktis is synthesized at 0 +/- 2 degrees C by the wild strain. This heat-labile alpha-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the described expression system, a compromise between enzyme stability and E. coli growth rate is reached at 18 degrees C.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Feller, Georges ; Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie
Le Bussy, O.
Gerday, Charles ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Language :
English
Title :
Expression of Psychrophilic Genes in Mesophilic Hosts: Assessment of the Folding State of a Recombinant Alpha-Amylase
Publication date :
March 1998
Journal title :
Applied and Environmental Microbiology
ISSN :
0099-2240
eISSN :
1098-5336
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
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