[en] Alpha-Amylase from the antarctic psychrophile Altermonas haloplanktis is synthesized at 0 +/- 2 degrees C by the wild strain. This heat-labile alpha-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the described expression system, a compromise between enzyme stability and E. coli growth rate is reached at 18 degrees C.
Disciplines :
Biochimie, biophysique & biologie moléculaire
Auteur, co-auteur :
Feller, Georges ; Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie
Le Bussy, O.
Gerday, Charles ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Langue du document :
Anglais
Titre :
Expression of Psychrophilic Genes in Mesophilic Hosts: Assessment of the Folding State of a Recombinant Alpha-Amylase
Date de publication/diffusion :
mars 1998
Titre du périodique :
Applied and Environmental Microbiology
ISSN :
0099-2240
eISSN :
1098-5336
Maison d'édition :
American Society for Microbiology, Washington, Etats-Unis - District de Columbia
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