Article (Scientific journals)
Mineralization kinetics in murine trabecular bone quantified by time-lapsed in vivo micro-computed tomography
Lukas, Carolin; Ruffoni, Davide; Lambers, Floor M. et al.
2013In BONE, 56 (1), p. 55-60
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Keywords :
Time-lapsed micro-CT; Bone formation; Bone resorption; Mineralization kinetics; Mechanical loading
Abstract :
[en] Trabecular bone is a highly dynamic tissue due to bone remodeling, mineralization and demineralization. The mineral content and its spatial heterogeneity are main contributors to bone quality. Using time-lapsed in vivo micro-computed tomography (micro-CT), it is now possible to resolve in three dimensions where bone gets resorbed and deposited over several weeks. In addition, the gray values in the micro-CT images contain quantitative information about the local tissue mineral density (TMD). The aim of this study was to measure how TMD increases with time after new bone formation and how this mineralization kinetics is influenced by mechanical stimulation. Our analysis of changes in TMD was based on an already reported experiment on 15-week-old female mice (C57BL/6), where in one group the sixth caudal vertebra was mechanically loaded with 8 N, while in the control group no loading was applied. Comparison of two consecutive images allows the categorization of bone into newly formed, resorbed, and quiescent bone for different time points. Gray values of bone in these categories were compared layer-wise to minimize the effects of beam hardening artifacts. Quiescent bone in the control group was found to mineralize with a rate of 8 +/- 1 mgHA/cm(3) per week, which is about half as fast as observed for newly formed bone. Mechanical loading increased the rate of mineral incorporation by 63% in quiescent bone. The week before bone resorption, demineralization could be observed with a drop of TMD by 36 +/- 4 mgHA/cm(3) in the control and 34 +/- 3 mgHA/cm(3) in the loaded group. In conclusion, this study shows how time-lapsed in vivo micro-CT can be used to assess changes in TMD of bone with high spatial and temporal resolution. This will allow a quantification of how bone diseases and pharmaceutical interventions influence not only microarchitecture of trabecular bone, but also its material quality. (C) 2013 Elsevier Inc. All rights reserved.
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Lukas, Carolin;  ETH, Inst Biomech, Zurich, Switzerland.
Ruffoni, Davide  ;  Université de Liège - ULiège > Département d'aérospatiale et mécanique > Mécanique des matériaux
Lambers, Floor M.;  ETH, Inst Biomech, Zurich, Switzerland.
Schulte, Friederike A.;  ETH, Inst Biomech, Zurich, Switzerland.
Kuhn, Gisela;  ETH, Inst Biomech, Zurich, Switzerland.
Kollmannsberger, Philip;  Max Planck Inst Colloids & Interfaces, Dept Biomat, D-14424 Potsdam, Germany.
Weinkamer, Richard;  Max Planck Inst Colloids & Interfaces, Dept Biomat, D-14424 Potsdam, Germany.
Mueller, Ralph;  ETH, Inst Biomech, Zurich, Switzerland.
Language :
English
Title :
Mineralization kinetics in murine trabecular bone quantified by time-lapsed in vivo micro-computed tomography
Publication date :
2013
Journal title :
BONE
ISSN :
8756-3282
eISSN :
1873-2763
Publisher :
Elsevier Science Inc, New York, United States - New York
Volume :
56
Issue :
1
Pages :
55-60
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 January 2014

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