[en] The GTP-binding proteins RhoA, Cdc42 and Rac1 regulate the organization and turnover of the cytoskeleton and cell-matrix adhesions, structures bridging cells to their support, and translating forces, external or generated within the cell. To investigate the specific requirements of Rho GTPases for biomechanical activities of clonal cell populations, we compared side-by-side stable lines of human fibroblasts expressing constitutively active (CA) RhoA, Cdc42 or Rac1. There was no marked effect of any CA GTPase on cell adhesion to different extracellular matrix proteins. Cell spreading was CA Rho GTPase specific and independent of the extracellular matrix proteins allowing adhesion. Mechanical properties were dramatically restricted by CA RhoA on bi- and in tri-dimensional surroundings, were boosted by CA Rac1 on bi-dimensional surroundings only, and were not or marginally affected by CA Cdc42. In conclusion, the action of Rho GTPases appears to depend on the task cells are performing.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Zhang, Z.-G.; University of Cologne > Center for Biochemistry, Center for Molecular Medecine
Lambert, Charles ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs
Servotte, S.; Université de Liège > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs
Chometon, G.; University of Cologne > Center for Biochemistry
Eckes, B.; University of Cologne > Department of Dermatology
Krieg, T.; University of Cologne > Department of Dermatology > Center for Biochemistry
Lapiere, C. M.; Laboratoire de Biologie des Tissus Conjonctifs
Nusgens, Betty ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs
Aumailley, M.; University of Cologne > Center for Biochemistry, Center for Molecular Medicine
Language :
English
Title :
Effects of constitutively active GTPases on fibroblast behavior.
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