[en] The incorporation efficiency of PTX was higher with the nanoprecipitation technique. The release behavior of PTX exhibited a biphasic pattern characterized by an initial burst release followed by a slower and continuous release. The in vitro anti-tumoral activity was assessed using the Human Cervix Carcinoma cells (HeLa) by the MTT test and was compared to the commercial formulation Taxol® and to Cremophor® EL. When exposed to 25 µg/ml of PTX, the cell viability was lower for PTX-loaded nanoparticles than for Taxol® (IC50 5.5 vs 15.5 µg/ml). Flow cytometry studies showed that the cellular uptake of PTX-loaded nanoparticles was concentration and time dependent. Exposure of HeLa cells to Taxol® and PTX-loaded nanoparticles induced the same percentage of apoptotic cells. PTX-loaded nanoparticles showed greater tumor growth inhibition effect in vivo on TLT tumor, compared with Taxol®. Therefore, PTX-loaded nanoparticles may be considered as an effective anticancer drug delivery system for cancer chemotherapy.
Research Center/Unit :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Materials science & engineering Chemistry
Author, co-author :
Danhier, Fabienne; Université catholique de Louvain (UCL) > Unité de Pharmacie Galénique
Lecouturier, Nathalie; Université catholique de Louvain (UCL) > Unité de Pharmacie Galénique
Vroman, Benoît; Université catholique de Louvain (UCL) > Unité de Pharmacie Galénique
Jérôme, Christine ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Marchand-Brynaert, Jacqueline; Universté catholique de Louvain (UCL) > Unité de Chimie Organique et Médicinale
Feron, Olivier; Université catholique de Louvain (UCL) > Laboratoire de Pharmacologie et de Thérapeutique
Préat, Véronique; Université catholique de Louvain (UCL) > Unité de Pharmacie Galénique
Language :
English
Title :
Paclitaxel-loaded PEGylated PLGA-based nanoparticles: in vitro and in vivo evaluation
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