Cold-Adapted Enzymes: From Fundamentals to Biotechnology

[en] Psychrophilic enzymes produced by cold-adapted microorganisms display a high catalytic efficiency and are most often, if not always, associated with high thermosensitivity. Using X-ray crystallography, these properties are beginning to become understood, and the rules governing their adaptation to cold appear to be relatively diverse. The application of these enzymes offers considerable potential to the biotechnology industry, for example, in the detergent and food industries, for the production of fine chemicals and in bioremediation processes.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Gerday, Charles ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)

Aittaleb, Mohamed

Bentahir, Mostafa

Chessa, Jean-Pierre

Claverie, Paule

Collins, Tony

D'Amico, Salvino ; Université de Liège - ULiège > GIGA-Research

Dumont, Joëlle

Garsoux, Geneviève

Georlette, Daphné

More authors (4 more)

Language :

English

Title :

Cold-Adapted Enzymes: From Fundamentals to Biotechnology

Publication date :

March 2000

Journal title :

Trends in Biotechnology

ISSN :

0167-7799

eISSN :

1879-3096

Publisher :

Elsevier, Netherlands

Volume :

Issue :

Pages :

103-7

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 January 2010

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580

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553

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469

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638

Bibliography

1 Gerday C.et al. Psychrophilic enzymes: a thermodynamic challenge. Biochim. Biophys. Acta. 1342:1997;119-131.
2 Russell N.J. Molecular adaptations in psychrophilic bacteria: potential for biotechnological applications. Adv. Biochem. Eng. Biotechnol. 61:1998;1-21.
3 Delille D., Bouvy M. Relationship between viable and direct bacteriological counts in southern polar marine waters. Vie milieu. 40:1990;281-284.
4 Feller G.et al. Temperature dependence of growth, enzyme secretion and activity of psychrophilic Antarctic bacteria. Appl. Microbiol. Biotechnol. 41:1994;477-479.
5 Tsigos I.et al. Purification and characterization of an alcohol dehydrogenase from the Antarctic psychrophile Moraxella sp. TAE 123. Eur. J. Biochem. 254:1998;356-362.
6 Feller G.et al. Purification, characterization and nucleotide sequence of the thermolabile α-amylase from the Antarctic psychrotroph Alteromonas haloplanctis A23. J. Biol. Chem. 267:1992;5217-5221.
7 Sun K.et al. Properties of aspartate transcarbamylase from TAD1 a psychrophilic bacterial strain. FEMS Microbiol. Lett. 164:1998;375-382.
8 Villeret V.et al. Preliminary crystal structure determination of the alkaline protease from the Antarctic psychrophile Pseudomonas aeruginosa. Protein Sci. 6:1997;2462-2464.
9 Gerike V.et al. Sequencing and expression of the gene encoding a cold-active citrate synthase from an Antarctic bacterium strain DS2-3R. Eur. J. Biochem. 248:1997;49-57.
10 Feller G.et al. The class C β-lactamase from the Antarctic psychrophile Psychrobacter immobilis A5. Eur. J. Biochem. 244:1997;186-191.
11 Kim S.Y.et al. Structural basis of cold adaptation. Sequence, biochemical properties and crystal structure of malate dehydrogenase from a psychrophile Aquaspirillium arcticum. J. Biol. Chem. 274:1999;11761-11767.
12 Narinx E.et al. Subtilisin from psychrophilic Antarctic bacteria: characterization and site-directed mutagenesis of residues possibly involved in the adaptation to cold. Protein Eng. 11:1997;1271-1279.
13 Alvarez M.et al. Triose phosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus. J. Biol. Chem. 273:1998;2199-2206.
14 Petrescu, I. et al. The xylanase from the Antarctic yeast Cryptococcus adeliae. Extremophiles (in press).
15 Tutino M.L.et al. Aspartate aminotransferase from Moraxella TAC 125: an unusual psychrophilic enzyme. Margesin R., Schinner F. Cold-adapted Organisms. 1999;305-316 Springer.
16 Zavodsky P.et al. Adjustment of conformational flexibility is a key event in the thermal adaptation of proteins. Proc. Natl. Acad. Sci. U. S. A. 95:1998;7406-7411.
17 Shoichet B.K.et al. A relationship between protein stability and protein function. Proc. Natl. Acad. Sci. U. S. A. 92:1995;452-456.
18 Vihinen M. Relationship of protein flexibility to thermostability. Protein Eng. 1:1987;477-480.
19 Fontes G.M.et al. Evidence of a general role for non-catalytic thermostabilizing domains in xylanases from thermophilic bacteria. Biochem. J. 307:1995;151-158.
20 Van den Burg B.et al. Engineering an enzyme to resist boiling. Proc. Natl. Acad. Sci. U. S. A. 95:1998;2056-2060.
21 Aghajari N.et al. Crystallization and preliminary X-ray diffraction studies of α-amylase from the Antarctic psychrophile Alteromonas haloplanctis A23. Protein Sci. 5:1996;2128-2129.
22 Russell R.J.M.et al. Structural adaptations of the cold-active citrate synthases from an Antarctic bacterium. Structure. 6:1998;351-361.
23 Aghajari N.et al. Crystal structures of the psychrophilic α-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor. Protein Sci. 7:1998;564-572.
24 Aghajari N.et al. Structures of the psychrophilic Alteromonas haloplanctis α-amylase give insights into cold adaptation at molecular level. Structure. 6:1998;1503-1506.
25 Feller G.et al. Thermodynamic stability of a cold-active α-amylase from the Antarctic bacterium Alteromonas haloplanctis. Biochemistry. 38:1999;4613-4619.
26 Beadle B.M.et al. Comparing the thermodynamic stabilities of a related thermophilic and mesophilic enzyme. Biochemistry. 38:1999;2570-2576.
27 Sproessler B.G. Milling and baking. Nagodawithana T., Reed G. Enzymes in Food Processing. 1993;293-320 Academic Press.
28 Timmis K.N., Pieper D.H. Bacteria designed for bioremediation. Trends Biotechnol. 17:1999;201-204.
29 Margesin R., Schinner F. Biodegradation of organic pollutants at low temperatures. Margesin R., Schinner F. Biotechnological Applications of Cold-adapted Organisms. 1999;271-289 Springer.
30 Margesin R., Schinner F. Efficiency of indigenous and inoculated cold-adapted soil microorganisms for biodegradation of diesel oil in alpine soils. Appl. Environ. Microbiol. 63:1997;2660-2664.
31 Margesin R., Schinner F. Low-temperature bioremediation of a waste water contaminated with anionic surfactant and fuel oil. Appl. Microbiol. Biotechnol. 49:1998;482-486.
32 Bell G.et al. Biocatalyst behaviour in low-water systems. Trends Biotechnol. 13:1995;468-473.
33 Partridge J.et al. Practical route to high activity enzyme preparations for synthesis in organic media. Chem. Commun. 1998;841-842.
34 Zhao H., Arnold F. Directed evolution converts subtilisin into a functional equivalent of thermilase. Protein Eng. 12:1999;47-53.