Article (Scientific journals)
Improved computer-assisted analysis of the global lymphatic network in human cervical tissues.
Balsat, Cédric; Signolle, Nicolas; GOFFIN, Frédéric et al.
2014In Modern Pathology, 27 (6), p. 887-98
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Keywords :
cancer; cervix; computerized;; distribution; lymphatic;; metastasis
Abstract :
[en] Lymphatic dissemination is a key event in cervical cancer progression and related tumor lymphatic markers are viewed as promising prognostic factor of nodal extension. However, validating such parameters requires an objective characterization of the lymphatic vasculature. Here, we performed a global analysis of the lymphatic network using a new computerized method applied on whole uterine cervical digital images. Sixty-eight cases of cervical neoplasia (12 CIN3, 10 FIGO stage 1A and 46 stage IB1) and 10 cases of normal cervical tissue were reacted with antibodies raised against D2-40, D2-40/p16 and D2-40/Ki67. Immunostained structures were automatically detected on whole slides. The lymphatic vessel density (D2-40), proliferating lymphatic vessel density (D2-40/ki67) and spatial lymphatic distribution in respect to the adjacent epithelium were assessed from normal cervix to early cervical cancer and correlated with lymphovascular space invasion and lymph node status. Prominent lymphatic vessel density and proliferating lymphatic vessel density are detected under the transformation zone of benign cervix and no further increase is noted during cancer progression. Notably, a shift of lymphatic vessel distribution toward the neoplastic edges is detected. In IB1 cervical cancer, although intra- and peritumoral lymphatic vessel density are neither correlated with lymphovascular space invasion nor with lymph node metastasis, a specific spatial distribution with more lymphatic vessels in the vicinity of tumor edges is predictive of lymphatic dissemination. Herein, we provide a new computerized method suitable for an innovative detailed analysis of the lymphatic network. We show that the transformation zone of the benign cervix acts as a baseline lymphangiogenic niche before the initiation of neoplastic process. During cancer progression, this specific microenvironment is maintained with lymphatic vessels even in closer vicinity to tumor cells.Modern Pathology advance online publication, 6 December 2013; doi:10.1038/modpathol.2013.195.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Balsat, Cédric ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Signolle, Nicolas
GOFFIN, Frédéric ;  Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
DELBECQUE, Katty ;  Centre Hospitalier Universitaire de Liège - CHU > Anatomie pathologique
Plancoulaine, Benoit
Sauthier, Philippe
Samouelian, Vanessa
BELIARD, Aude ;  Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Sénologie -Obstétrique CHBA
Munaut, Carine  ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Blacher, Silvia ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Noël, Agnès ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Kridelka, Frédéric ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie-Obstétrique
More authors (3 more) Less
Language :
English
Title :
Improved computer-assisted analysis of the global lymphatic network in human cervical tissues.
Publication date :
2014
Journal title :
Modern Pathology
ISSN :
0893-3952
eISSN :
1530-0285
Publisher :
Lippincott Williams & Wilkins, Hagerstown, United States - Maryland
Volume :
27
Issue :
6
Pages :
887-98
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 January 2014

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