Article (Scientific journals)
The importance of carbonic anhydrase II in red blood cells during exposure of chicken embryos to CO2.
Everaert, Nadia; Willemsen, H.; Hulikova, A. et al.
2010In Respiratory Physiology and Neurobiology, 172 (3), p. 154-61
Peer Reviewed verified by ORBi
 

Files


Full Text
Everaert et al 2010 Resp Physiol Neurobiol.pdf
Publisher postprint (422.83 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Adaptation, Physiological/physiology; Animals; Blotting, Western; Body Weight/physiology; Carbon Dioxide/pharmacology; Carbonic Anhydrase II/blood; Chick Embryo; Embryonic Development; Erythrocytes/enzymology; Potassium/blood
Abstract :
[en] The importance of carbonic anhydrase (CA) during exposure of chicken embryos to CO(2) during the second half of incubation was investigated. The protein abundance and activity of CAII in erythrocytes was significantly higher in CO(2)-exposed embryos compared to normal conditions. Daily injections of acetazolamide (ATZ), an inhibitor of CA, increased blood P(CO2) and decreased blood pH in both control and CO(2)-incubated embryos. ATZ increased blood bicarbonate concentration in embryos exposed to normal atmosphere and in day-12 embryos exposed to high CO(2). The tendency of an increased blood potassium concentration in ATZ-injected embryos under standard atmospheric conditions might indicate that protons were exchanged with intracellular potassium. However, there was no evidence for such an exchange in CO(2)-incubated ATZ-treated embryos. This study shows for the first time that chicken embryos adapt to CO(2) during the second half of incubation by increasing CAII protein expression and function in red blood cells. This response may serve to "buffer" elevated CO(2) levels.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Everaert, Nadia ;  KU Leuven > Department of Biosystems > Division Livestock-Nutrition-Quality
Willemsen, H.
Hulikova, A.
Brown, H.
Decuypere, E.
Swietach, P.
Bruggeman, V.
Language :
English
Title :
The importance of carbonic anhydrase II in red blood cells during exposure of chicken embryos to CO2.
Publication date :
2010
Journal title :
Respiratory Physiology and Neurobiology
ISSN :
1569-9048
eISSN :
1878-1519
Publisher :
Elsevier, Netherlands
Volume :
172
Issue :
3
Pages :
154-61
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright 2010 Elsevier B.V. All rights reserved.
Available on ORBi :
since 18 December 2013

Statistics


Number of views
31 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
11
Scopus citations®
without self-citations
9
OpenCitations
 
10

Bibliography


Similar publications



Contact ORBi