Effects of heat exposure on Akt/S6K1 signaling and expression of genes related to protein and energy metabolism in chicken (Gallus gallus) pectoralis major muscle.
Animals; Avian Proteins/genetics/metabolism; Blood Glucose/analysis; Chickens/genetics/growth & development/metabolism; Energy Metabolism; Gene Expression; Hot Temperature; Insulin/blood; Male; Pectoralis Muscles/growth & development/metabolism; Proto-Oncogene Proteins c-akt/metabolism; Ribosomal Protein S6 Kinases, 70-kDa/metabolism; Signal Transduction
Abstract :
[en] In order to improve understanding of the heat-induced changes in muscle growth, we determined the expression of genes related to protein and energy metabolism in the pectoralis major muscle of chickens. We also explored the protein kinase B (PKB also called Akt)/p70 S6 kinase (S6K1)/S6 pathway that mediates anabolic signals thereby regulating metabolism and hypertrophic/atrophic balance. Four-week-old chickens were exposed to 32 or 22 degrees C for 1 week. Chickens from both groups were then fasted for 16 h or left fed, and submitted to an oral administration of glucose-arginine to induce an anabolic response (30-min treatment) or left untreated. High ambient temperature and the associated decrease in feed intake modified the expression of certain energy-related genes (e.g. -40% for PGC-1alpha) and protein metabolism (e.g. about +80% for atrogin-1), but the expression of several muscle metabolism-related genes considered here was unchanged. The capacity for muscle protein synthesis, i.e. RNA/protein ratio, was reduced in warm conditions (approximately -20%). Slightly lower activation of S6 induced by glucose-arginine treatment was found at 32 degrees C compared to 22 degrees C, which might indicate somewhat lower efficiency of mRNA translation. Analysis of glucose/insulin balance suggested changes in glucose metabolism under heat exposure. However, this remains to be characterized.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Boussaid-Om Ezzine, S.
Everaert, Nadia ; KU Leuven > Department of Biosystems > Division Livestock-Nutrition-Quality
Metayer-Coustard, S.
Rideau, N.
Berri, C.
Joubert, R.
Temim, S.
Collin, A.
Tesseraud, S.
Language :
English
Title :
Effects of heat exposure on Akt/S6K1 signaling and expression of genes related to protein and energy metabolism in chicken (Gallus gallus) pectoralis major muscle.
Publication date :
2010
Journal title :
Comparative Biochemistry and Physiology. Part B, Biochemistry and Molecular Biology
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