Reference : Mitochondrial comparative proteomics: Strenghts and Pitfalls
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Mitochondrial comparative proteomics: Strenghts and Pitfalls
Mathy, Grégory [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
Sluse, Francis mailto [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
Biochimica et Biophysica Acta-Bioenergetics
Elsevier Science
Yes (verified by ORBi)
[en] Mitochondria ; Comparative proteomics
[en] In this review, we describe the various techniques available to carry out valid comparative proteomics, their advantages and their disadvantages according to the goal of the research. Two-dimensional electrophoresis and 2D-DIGE are compared to shotgun proteomics and SILE. We give our opinion on the best fields of application in the domain of comparative proteomics. We emphasize the usefulness of these new tools, providing mass data to study physiology and mitochondrial plasticity when faced with a specific mitochondrial insufficiency or exogenic stress. We illustrate the subject with results obtained in our laboratory specifying the importance of an approach of comparative proteomics combined from mitochondria and from the cell, which makes it possible to obtain important information on the status of the mitochondrial function at the cellular level. Finally, we draw attention to the dangers of the extrapolation of proteomic data to metabolic flows which requires the greatest care
Fonds de la Recherche Fondamentale Collective d’Initiative des Chercheurs - FRFC
Researchers ; Students

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