Carbohydrates; non-protein thiols; plant proteomics; water relations; proline; photosynthetic-related parameters; micro-PIXE; LA-ICP-MS; heavy metals; EXAFS
Abstract :
[en] Cadmium and zinc share many similar physiochemical properties, but their compartmentation, complexation and impact on other mineral element distribution in plant tissues may drastically differ. In this study, we address the impact of 10-μM Cd or 50-μM Zn treatment on ion distribution in leaves of a metallicolous population of the non-hyperaccumulating species Zygophyllum fabago at tissue and cell level, and the consequences on the plant response through a combined physiological, proteomic and metabolite approach. Micro-proton induced X-ray emission and laser ablation inductively coupled mass spectrometry analyses indicated hot spots of Cd concentrations in the vicinity of vascular bundles in response to Cd treatment, essentially bound to S-containing compounds as revealed by extended X-Ray absorption fine structure and non-protein thiol compounds analyses. A preferential accumulation of Zn occurred in vascular bundle and spongy mesophyll in response to Zn treatment, and was mainly bound to O/N-ligands. Leaf proteomics and physiological status evidenced a protection of photosynthetically active tissues and the maintenance of cell turgor through specific distribution and complexation of toxic ions, reallocation of some essential elements, synthesis of proteins involved in photosynthetic apparatus or C-metabolism, and metabolite synthesis, with some specificities regarding the considered heavy metal treatment.
LEFÈVRE, Isabelle; Université Catholique de Louvain - UCL > Earth and Life Institute – Agronomy (ELI-A), > Groupe de Recherche en Physiologie végétale (GRPV)
VOGEL‐MIKUŠ, Katarina; University of Ljubljana (Slovenia) > Department of Biology
JEROMEL, Luka; Jožef Stefan Institute (Lubjanja, Slovenia)
VAVPETIČ, Primož; Jožef Stefan Institute (Lubjanja, Slovenia)
PLANCHON, Sébastien; Centre de Recherche Public-Gabriel Lippmann (Luxembourg) > Department of Environment and Agrobiotechnologies (EVA)
ARČON, Iztok; University of Nova Gorica (Slovenia)
VAN ELTEREN, Ohannes T.; National Institute of Chemistry (Slovenia) > Analytical Chemistry Laboratory
Lepoint, Gilles ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanologie
Gobert, Sylvie ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanologie
Renaut, Jenny; Centre de Recherche Public-Gabriel Lippmann (Luxembourg) > Department of Environment and Agrobiotechnologies (EVA)
Pelicon, Primoz; Jožef Stefan Institute (Lubjanja, Slovenia)
Lutts, Stanley; Université Catholique de Louvain - UCL > Earth and Life Institute – Agronomy (ELI-A), > Physiologie vegetale
Language :
English
Title :
Differential cadmium and zinc distribution in relation to their physiological impact in the leaves of the accumulating Zygophyllum fabago L
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